Kudryakova Irina, Afoshin Alexey, Tarlachkov Sergey, Pavlenko Sofya, Suzina Natalia, Shishkova Nina, Leontyevskaya Elena, Leontyevskaya Natalia
Laboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC PSCBR, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, Russia, 142290.
Lomonosov Moscow State University, Leninskiye Gory, Moscow, Russia, 119899.
Sci Rep. 2025 Jul 1;15(1):22257. doi: 10.1038/s41598-025-07691-2.
One of the most pressing issues in modern biomedicine is the search for new antimicrobial agents - antibiotics, peptides, bacteriolytic enzymes. This study, using transcriptomic and proteomic approaches, identified a new extracellular bacteriolytic enzyme of Lysobacter capsici XL1 - the amidase Ami. The enzyme was isolated and characterized. Ami was found to hydrolyze the amide bond between the carbohydrate and peptide fragments in bacterial peptidoglycans of chemotypes A1γ, A3α, and A4α. Ami lysed live target cells of opportunistic bacteria Micrococcus luteus Ac-2230, Bacillus cereus 217, Staphylococcus aureus 209P, Enterococcus faecium FS86, phytopathogenic bacteria Bacillus megaterium MS941, Curtobacterium flaccumfaciens pv. flaccumfaciens, and pathogenic bacteria of various strains of B. anthracis, including plasmid strains 71/12 and ΔAmes, as well as strains of B. cereus with hemolytic, lecithinase, and phosphatase activities. Thus, the bacteriolytic amidase Ami is a promising candidate for the development of next-generation antimicrobial drugs.
现代生物医学中最紧迫的问题之一是寻找新型抗菌剂——抗生素、肽、溶菌酶。本研究采用转录组学和蛋白质组学方法，鉴定出一种来自辣椒溶杆菌XL1的新型细胞外溶菌酶——酰胺酶Ami。该酶被分离并进行了特性分析。发现Ami能水解化学型A1γ、A3α和A4α细菌肽聚糖中碳水化合物和肽片段之间的酰胺键。Ami能裂解机会致病菌藤黄微球菌Ac-2230、蜡样芽孢杆菌217、金黄色葡萄球菌209P、粪肠球菌FS86、植物病原菌巨大芽孢杆菌MS941、萎蔫短小杆菌萎蔫致病变种，以及包括质粒菌株71/12和ΔAmes在内的各种炭疽芽孢杆菌菌株，还有具有溶血、卵磷脂酶和磷酸酶活性的蜡样芽孢杆菌菌株的活靶细胞。因此，溶菌酰胺酶Ami是开发下一代抗菌药物的有前景的候选物。
