非洲爪蟾卵母细胞中编码脑和肾上腺血管紧张素II受体的大小选择信使核糖核酸的表达

Expression of size-selected messenger RNA encoding the brain and adrenal gland angiotensin-II receptor in Xenopus laevis oocytes.

作者信息

Inestrosa N C, Cross D

机构信息

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Catholic University of Chile, Santiago.

出版信息

Arch Biol Med Exp. 1988 Dec;21(3-4):423-8.

PMID:3256274

Abstract

The expression of the rat angiotensin-II receptor has been studied in Xenopus oocytes. Poly(A)+ RNA isolated from the brain and adrenal gland was injected into oocytes, and the expression of the receptor in the oocyte plasma membrane was assayed by measuring the change in membrane potential in the presence of angiotensin-II. Expression of the angiotensin-II receptor was detected 1.0-1.5 days after messenger RNA (mRNA) injection, and the degree of membrane depolarization was proportional to the amount of mRNA injected. Ca+2 channel blockers inhibited the angiotensin-II-induced depolarization. The total mRNA was fractionated by preparative agarose gel electrophoresis and each fraction was assayed for its ability to induce angiotensin-II depolarization. The mRNA encoding the angiotensin-II receptor was found in a single fraction of 4.4 kilobases.

摘要

已在非洲爪蟾卵母细胞中研究了大鼠血管紧张素 II 受体的表达。从大脑和肾上腺分离出的聚腺苷酸加尾（Poly(A)+）RNA 被注入卵母细胞，通过测量血管紧张素 II 存在时膜电位的变化来测定卵母细胞质膜中受体的表达。在注射信使核糖核酸（mRNA）后 1.0 - 1.5 天检测到血管紧张素 II 受体的表达，并且膜去极化程度与注射的 mRNA 量成比例。钙离子通道阻滞剂抑制血管紧张素 II 诱导的去极化。通过制备性琼脂糖凝胶电泳对总 mRNA 进行分级分离，并对每个级分诱导血管紧张素 II 去极化的能力进行测定。发现编码血管紧张素 II 受体的 mRNA 存在于一个 4.4 千碱基的单一级分中。