Matute C, Pulakat L, Río C, Valcárcel C, Miledi R
Department of Psychobiology, University of California, Irvine 92717.
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3774-8. doi: 10.1073/pnas.91.9.3774.
The existence and the properties of angiotensin II receptors in the adult bovine and human corpus callosum (CC) were investigated by using Xenopus oocytes and primary glial cell cultures. In oocytes injected with CC mRNA, angiotensin II elicited oscillatory Cl- currents due to activation of the inositol phosphate/Ca(2+)-receptor-channel coupling system. The receptors expressed in oocytes and in CC cultures were pharmacologically similar to the AT1 receptor type as assayed by binding. Northern blot analysis and in situ hybridization studies in sections from CC and in glial cultures revealed that the receptors were molecularly related to the AT1 receptor and that they were present in astrocytes. In these cells, activation of the receptors with angiotensin II increased de novo DNA synthesis, promoted the release of aldosterone, and induced c-Fos expression. These findings indicate that CC astrocytes possess functional AT1 receptors that participate in various physiological processes.
利用非洲爪蟾卵母细胞和原代胶质细胞培养物，对成年牛和人类胼胝体（CC）中血管紧张素II受体的存在及特性进行了研究。在注射了CC mRNA的卵母细胞中，血管紧张素II由于肌醇磷酸/Ca(2+) -受体-通道偶联系统的激活而引发振荡性Cl-电流。通过结合试验测定，卵母细胞和CC培养物中表达的受体在药理学上与AT1受体类型相似。对CC切片以及胶质细胞培养物进行的Northern印迹分析和原位杂交研究表明，这些受体在分子水平上与AT1受体相关，且存在于星形胶质细胞中。在这些细胞中，用血管紧张素II激活受体可增加DNA的从头合成，促进醛固酮的释放，并诱导c-Fos表达。这些发现表明，CC星形胶质细胞具有参与各种生理过程的功能性AT1受体。
