Jiang Xin, Zhang Jian, Xu Lin, Wang Weie, Du Juan, Qu Minghuan, Han Xiaoxia, Yang Libin, Zhao Bing
College of Pharmacy, Jiamusi University, Jiamusi 154007, People's Republic of China.
State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 15;240:118589. doi: 10.1016/j.saa.2020.118589. Epub 2020 Jun 11.
Methotrexate (MTX) is a drug with broad-spectrum antitumor activity that is of great importance in therapeutic drug monitoring applications. In this essay, the two-step modified concentrated Ag colloid with the assistance of KF and MgSO was used as the SERS active substrate for the ultrasensitive detection of MTX and its commercial formulations (tablets). It can be found that the two-step modification of the samples is a crucial procedure to remove the by-products in the synthesis of Ag colloid and further concentrate the Ag colloid. Under the optimal detection conditions, the minimum detection concentration of MTX is 1 × 10 mol/L. And, there is a good linear relationship over a wide concentration range of 1 × 10-1 × 10 mol/L. The labelled amounts of the two manufacturers of MTX commercial tablets are in the range of 96.4-104.3% with the RSDs between 1.8% and 3.5% by this method, which are in accordance with the methodological requirements. These results prove that the proposed SERS method exhibits a good reproducibility and ultra-high sensitivity for the detection of the antitumor drug MTX.
甲氨蝶呤（MTX）是一种具有广谱抗肿瘤活性的药物，在治疗药物监测应用中具有重要意义。在本文中，以两步法在KF和MgSO辅助下修饰的浓缩银胶体作为表面增强拉曼光谱（SERS）活性基底，用于超灵敏检测MTX及其市售制剂（片剂）。可以发现，样品的两步修饰是去除银胶体合成过程中的副产物并进一步浓缩银胶体的关键步骤。在最佳检测条件下，MTX的最低检测浓度为1×10⁻⁹mol/L。并且，在1×10⁻⁷ - 1×10⁻⁹mol/L的宽浓度范围内存在良好的线性关系。采用该方法测定的两个厂家MTX市售片剂的标示量在96.4% - 104.3%之间，相对标准偏差（RSD）在1.8% - 3.5%之间，符合方法学要求。这些结果证明，所提出的SERS方法在检测抗肿瘤药物MTX时具有良好的重现性和超高灵敏度。
