Liu Dan, Ji Jiahui, Guo Xinran, Gou Sanhu, Chen Xinyue
School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
Micromachines (Basel). 2023 Jan 30;14(2):350. doi: 10.3390/mi14020350.
In this paper, silver nanoparticles (AgNPs) were successfully green-synthesized for the first time using Hedysarum polysaccharide (HPS) as a reducing agent, stabilizer, and modifier (HPS-AgNP). Thiamazole could induce the aggregation of HPS-AgNPs in the residue on a cellulose membrane. A syringe paper-based analytical device was creatively established to ensure the tightness, stability, and good repeatability of the test. The color information remaining on the cellulose membrane was converted into gray values using ImageJ software. Hence, the linear regression curve for thiamazole was established as y = 1 + 0.179x with a detection limit (LOD) of 24.6 nM in the relatively wide range of 0.1~10 μM. This syringe paper-based analytical device was successfully applied to the biological samples.
在本文中，首次成功地以岩黄芪多糖（HPS）作为还原剂、稳定剂和改性剂绿色合成了银纳米颗粒（AgNPs）（HPS-AgNP）。硫代咪唑可诱导纤维素膜上残留的HPS-AgNPs聚集。创新性地建立了一种基于注射器的纸质分析装置，以确保测试的密封性、稳定性和良好的重复性。使用ImageJ软件将纤维素膜上残留的颜色信息转换为灰度值。因此，硫代咪唑的线性回归曲线为y = 1 + 0.179x，在0.1~10 μM的较宽范围内检测限（LOD）为24.6 nM。这种基于注射器的纸质分析装置已成功应用于生物样品。
