ICAR-Central Potato Research Institute, Shimla, 171001, Himachal Pradesh, India.
ICAR-Central Potato Research Institute, Shimla, 171001, Himachal Pradesh, India.
Plant Physiol Biochem. 2020 Sep;154:171-183. doi: 10.1016/j.plaphy.2020.05.041. Epub 2020 Jun 9.
Nitrogen (N) is an important nutrient for plant growth. However, its excess application leads to environmental damage. Hence, improving nitrogen use efficiency (NUE) of plant is one of the plausible options to solve the problems. Aim of this study was to identify candidate genes involved in enhancing NUE in potato cv. Kufri Gaurav (N efficient). Plants were grown in aeroponic with two contrasting N regimes (low N: 0.75 mM, and high N: 7.5 mM). Higher NUE in Kufri Gaurav was observed in low N based on the parameters like NUE, NUpE (N uptake efficiency), NUtE (N utilization efficiency) and AgNUE (agronomic NUE). Further, global gene expression profiles in root, leaf and stolon tissues were analyzed by RNA-sequencing using Ion Proton™ System. Quality data (≥Q20) of 2.04-2.73 Gb per sample were mapped with the potato genome. Statistically significant (P ≤ 0.05) differentially expressed genes (DEGs) were identified such as 176 (up-regulated) and 30 (down-regulated) in leaves, 39 (up-regulated) and 105 (down-regulated) in roots, and 81 (up-regulated) and 694 (down-regulated) in stolons. The gene ontology (GO) terms like metabolic process, cellular process and catalytic activity were predominant. Our RT-qPCR analysis confirmed the gene expression profiles of RNA-seq. Overall, we identified candidate genes associated with improving NUE such as superoxide dismutase, GDSL esterase lipase, probable phosphatase 2C, high affinity nitrate transporters, sugar transporter, proline rich proteins, transcription factors (VQ motif, SPX domain, bHLH) etc. Our findings suggest that these candidate genes probably play crucial roles in enhancing NUE in potato.
氮（N）是植物生长的重要营养物质。然而，过量施氮会导致环境破坏。因此，提高植物的氮利用效率（NUE）是解决这些问题的可行方案之一。本研究旨在鉴定参与提高马铃薯 cv. Kufri Gaurav（N 高效）氮利用效率的候选基因。植株在气培条件下，采用两种不同氮处理（低氮：0.75 mM，高氮：7.5 mM）。基于氮利用效率、NUpE（氮吸收效率）、NUtE（氮利用效率）和 AgNUE（农学氮利用效率）等参数，Kufri Gaurav 在低氮条件下表现出较高的 NUE。进一步通过 Ion Proton™ System 进行 RNA-seq 分析，研究了根、叶和匍匐茎组织的全局基因表达谱。每个样本的质量数据（≥Q20）为 2.04-2.73 Gb，与马铃薯基因组进行了比对。在叶片中鉴定到 176 个（上调）和 30 个（下调）、在根中鉴定到 39 个（上调）和 105 个（下调）、在匍匐茎中鉴定到 81 个（上调）和 694 个（下调）的差异表达基因（DEGs），差异表达基因在叶片、根和匍匐茎中分别达到了统计学意义（P ≤ 0.05）。GO 术语如代谢过程、细胞过程和催化活性占主导地位。我们的 RT-qPCR 分析证实了 RNA-seq 的基因表达谱。总的来说，我们鉴定到了与提高 NUE 相关的候选基因，如超氧化物歧化酶、GDSL 酯酶脂肪酶、可能的磷酸酶 2C、高亲和力硝酸盐转运蛋白、糖转运蛋白、脯氨酸丰富蛋白、转录因子（VQ 基序、SPX 结构域、bHLH）等。我们的研究结果表明，这些候选基因可能在提高马铃薯氮利用效率方面发挥着重要作用。
