Department of Neurology, the Second Affiliated Hospital of Nanchang University, Nanchang, China.
BMC Neurol. 2020 Jun 20;20(1):250. doi: 10.1186/s12883-020-01780-7.
Post-stroke depression (PSD) is a mood disorder characterized by depression and anhedonia caused by stroke. Metabolomics identified metabolites associated with PSD, but previous studies are based on gas chromatography (GC)/mass spectrometry (MS). This study aimed to perform a liquid chromatography (LC)-MS-based metabolomics study of the plasma metabolite profiles between patients with PSD and controls.
This was a prospective study of patients with stroke enrolled between July and December 2017 at the Second Affiliated Hospital of Nanchang University. Patients were grouped as Hamilton Depression Rating Scale > 7 (PSD) or < 7 (controls). Metabonomics profiling of plasma sampled was conducted by LC-MS. By combining multivariable and univariable statistical analyses, significant differential metabolites between the two groups were screened. The threshold for significant differences was VIP ≥1 and P < 0.05. LogFC is the logarithm of the mean ratio between the two groups.
There were no significant difference with respect to age, NIHSS score, and MMSE between the two groups (all P > 0.05). There were six differential metabolites between the PSD and stroke groups, of which three metabolites were increased and three were decreased. Compared with the control group, p-chlorophenylalanine (LogFC = 1.37, P = 0.03), phenylacetyl glutamine (LogFC = 0.21, P = 0.048), and DHA (LogFC = 0.77, P = 0.01) levels were higher in the PSD group, while betaine (trimethylglycine) (LogFC = - 0.79, P = 0.04), palmitic acid (LogFC = - 0.51, P = 0.001), and MHPG-SO (LogFC = - 2.37, P = 0.045) were decreased.
Plasma metabolomics showed that amino acid metabolism (phenylacetyl glutamine, p-chlorophenylalanine, trimethylglycine), lipid metabolism (DHA, palmitic acid, trimethylglycine), and oxidative stress (DHA, palmitic acid, trimethylglycine) were associated with PSD. These results could help to reveal the pathophysiological mechanism of PSD and eventually identify treatment targets.
卒中后抑郁（PSD）是一种以抑郁和快感缺失为特征的心境障碍，由卒中引起。代谢组学鉴定了与 PSD 相关的代谢物，但以前的研究基于气相色谱（GC）/质谱（MS）。本研究旨在对 PSD 患者和对照者的血浆代谢物图谱进行基于液相色谱（LC）-MS 的代谢组学研究。
这是一项 2017 年 7 月至 12 月在南昌大学第二附属医院进行的前瞻性卒中患者研究。根据汉密尔顿抑郁评定量表评分（HAMD）＞7（PSD）或＜7（对照组）对患者进行分组。对采集的血浆进行代谢组学分析，采用 LC-MS 进行分析。通过多变量和单变量统计分析相结合，筛选两组间有显著差异的差异代谢物。显著差异的阈值为 VIP≥1 和 P＜0.05。LogFC 是两组之间平均比值的对数。
两组在年龄、NIHSS 评分和 MMSE 方面无显著差异（均 P＞0.05）。PSD 组和卒中组之间有 6 个差异代谢物，其中 3 个代谢物增加，3 个代谢物减少。与对照组相比，PSD 组中 p-氯苯丙氨酸（LogFC=1.37，P=0.03）、苯乙酰谷氨酰胺（LogFC=0.21，P=0.048）和 DHA（LogFC=0.77，P=0.01）水平升高，而甜菜碱（三甲甘氨酸）（LogFC=-0.79，P=0.04）、棕榈酸（LogFC=-0.51，P=0.001）和 MHPG-SO（LogFC=-2.37，P=0.045）降低。
血浆代谢组学显示，氨基酸代谢（苯乙酰谷氨酰胺、p-氯苯丙氨酸、三甲甘氨酸）、脂质代谢（DHA、棕榈酸、三甲甘氨酸）和氧化应激（DHA、棕榈酸、三甲甘氨酸）与 PSD 相关。这些结果可能有助于揭示 PSD 的病理生理机制，并最终确定治疗靶点。
