Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, No. 1 Yixueyuan Road, Chongqing, China.
Institute of Life Sciences, Chongqing Medical University, No. 1 Yixueyuan Road, Chongqing, China.
Neurochem Res. 2022 Jul;47(7):2052-2063. doi: 10.1007/s11064-022-03596-y. Epub 2022 Apr 25.
Post-stroke depression (PSD) is the most common mental disorder in stroke survivors. However, its specific pathophysiology remains largely unknown. Previous studies suggested a role of hippocampus in PSD. Therefore, we conducted this study to investigate the lipid metabolic signatures in hippocampus of PSD rats. Here, the liquid chromatography mass spectrometry was used to identify the lipid metabolic signatures in the hippocampus of PSD, control and stroke rats. Then, correlations between behavior indices and differential lipid metabolites in PSD rats were explored. Pathway and enrichment analysis were further conducted to uncover the crucial metabolic pathways related to PSD. Finally, we found that the lipid metabolic phenotype in hippocampus of PSD rats was substantially different from that in control and stroke rats, and identified 50 key lipid metabolites that were significantly decreased in PSD rats. These differential metabolites were mainly involved in glycerophospholipid metabolism. Meanwhile, the sucrose preference and immobility time were found to be significantly positively and negatively, respectively, correlated with glycerophospholipid metabolites. The pathway and enrichment analysis showed that the glycerophospholipid metabolism, especially cardiolipin metabolism, was significantly disturbed in PSD rats. These results suggested that the down-regulated glycerophospholipids in hippocampus, especially cardiolipin, might participate in the pathophysiology of PSD. Our findings would be helpful for future exploring the pathophysiology of PSD.
脑卒中后抑郁（PSD）是脑卒中幸存者中最常见的精神障碍。然而，其具体的病理生理学仍然很大程度上未知。先前的研究表明海马在 PSD 中起作用。因此，我们进行了这项研究，以调查 PSD 大鼠海马中的脂质代谢特征。在这里，我们使用液相色谱-质谱联用技术来鉴定 PSD、对照和脑卒中大鼠海马中的脂质代谢特征。然后，我们探讨了 PSD 大鼠行为指标与差异脂质代谢物之间的相关性。进一步进行了通路和富集分析，以揭示与 PSD 相关的关键代谢通路。最后，我们发现 PSD 大鼠海马中的脂质代谢表型与对照和脑卒中大鼠有很大的不同，并且鉴定出 50 种在 PSD 大鼠中显著减少的关键脂质代谢物。这些差异代谢物主要涉及甘油磷脂代谢。同时，蔗糖偏好和不动时间分别与甘油磷脂代谢物呈显著正相关和负相关。通路和富集分析表明，PSD 大鼠中的甘油磷脂代谢，特别是心磷脂代谢，明显受到干扰。这些结果表明，海马中下调的甘油磷脂，特别是心磷脂，可能参与 PSD 的病理生理学。我们的发现有助于未来探索 PSD 的病理生理学。
