Horticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, PR China; National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), No. 120 Dongling Road, Shenhe District 110866, PR China; Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, No. 120 Dongling Road, Shenhe District 110866, PR China.
Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Bioscience and Biotechnology, No. 120 Dongling Road, Shenhe District 110866, PR China.
Plant Sci. 2020 Aug;297:110530. doi: 10.1016/j.plantsci.2020.110530. Epub 2020 May 27.
Dwarfing is a desirable agronomic trait in cultivation management. Dwarf plants are lodging-resistant, compact, and perform well under high-density planting. The use of dwarf genetic resources is one approach to improve crop yield. YABBY2b in tomato (Solanum lycopersicum) encodes a transcription factor that regulates plant height. In this study, we created YABBY2b knockout mutant lines, and the resulting yabby2b plants exhibited reduced height and smaller flowers and fruits. The RNA-seq analysis showed that 17 genes responding to gibberellin and auxin were differentially expressed. We hypothesized that indole-3-acetic acid-amido synthetase GH3.8 (GH3.8) played a crucial role in the resulting yabby2b dwarf phenotype. Further analysis showed that YABBY2b suppresses GH3.8 gene expression by directly binding to its promoter, and that this contributes to auxin-mediated repression of GH3.8. Moreover, the silencing of GH3.8 led to increased plant height. Combined, our data suggest that YABBY2b may positively regulate plant height in tomato by inhibiting the expression of growth suppressor GH3.8.
矮化是栽培管理中一种理想的农艺性状。矮化植物抗倒伏、紧凑，在高密度种植下表现良好。利用矮化遗传资源是提高作物产量的一种方法。番茄中的 YABBY2b 基因编码一个转录因子，它调节植物的高度。在这项研究中，我们创建了 YABBY2b 敲除突变体系，结果表明 yabby2b 植株的高度降低，花朵和果实变小。RNA-seq 分析表明，有 17 个响应赤霉素和生长素的基因表达存在差异。我们假设吲哚-3-乙酸酰胺合成酶 GH3.8（GH3.8）在产生的 yabby2b 矮化表型中起着关键作用。进一步的分析表明，YABBY2b 通过直接结合其启动子抑制 GH3.8 基因的表达，这有助于生长素介导的 GH3.8 抑制。此外，GH3.8 的沉默导致株高增加。综上所述，我们的数据表明，YABBY2b 可能通过抑制生长抑制剂 GH3.8 的表达，正向调控番茄的株高。
