Salinas Constanza, Espinosa Gabriel, Morales Natalia, Henríquez Claudio, Morán Gabriel, Gajardo Gonzalo, Uberti Benjamin
Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile; Instituto de Ciencias Clínicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
Instituto de Farmacología y Morfofisiología, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
Res Vet Sci. 2020 Oct;132:127-132. doi: 10.1016/j.rvsc.2020.06.013. Epub 2020 Jun 12.
Obesity is a highly prevalent condition in horses. Dysfunctional neutrophil activity has been reported in metabolically healthy obese humans, but minimal data exist regarding horses. The present study evaluated the effect of obesity on apoptosis, phagocytosis and oxidative burst activity of peripheral blood neutrophils from lean and obese non-insulin dysregulated horses. Seven lean (BCS, body condition score 4-6/9) and five obese (BCS 8-9) horses were enrolled in the study. All animals underwent two metabolic tests (OGT, oral glucose test; IRT, insulin response test) before their selection to ensure their metabolic status (non-insulin dysregulated). A single blood sample was obtained from each horse, and a discontinuous density gradient was carried out to isolate neutrophils. Phagocytosis, apoptosis and reactive oxygen species (ROS) production assays were performed for each animal. All statistical analyses were performed with unpaired two-tailed t-tests. Results indicate that neutrophils from obese non-insulin dysregulated horses have a significantly increased ROS production (P < .0001), with no changes observed on phagocytosis (P > .05) or apoptosis (P > .05) when compared to the control group. In conclusion, our results demonstrate that obesity per se, in absence of other endocrine disorders, alters neutrophil reactive oxygen species production. More research is needed to understand the role of obesity on the equine immune system of horses, and its role in the development of endocrine disorders.
肥胖在马匹中是一种高度普遍的状况。在代谢健康的肥胖人类中已报道存在功能失调的中性粒细胞活性，但关于马匹的此类数据极少。本研究评估了肥胖对非胰岛素失调的瘦马和肥胖马外周血中性粒细胞凋亡、吞噬作用及氧化爆发活性的影响。七匹瘦马（体况评分，BCS，4 - 6/9）和五匹肥胖马（BCS 8 - 9）被纳入该研究。所有动物在入选前均接受了两项代谢测试（口服葡萄糖测试，OGT；胰岛素反应测试，IRT），以确保其代谢状态（非胰岛素失调）。从每匹马采集一份血样，并进行不连续密度梯度离心以分离中性粒细胞。对每只动物进行吞噬作用、凋亡及活性氧（ROS）生成测定。所有统计分析均采用非配对双尾t检验。结果表明，与对照组相比，非胰岛素失调的肥胖马的中性粒细胞ROS生成显著增加（P <.0001），而吞噬作用（P >.05）或凋亡（P >.05）未观察到变化。总之，我们的结果表明，在不存在其他内分泌紊乱的情况下，肥胖本身会改变中性粒细胞的活性氧生成。需要更多研究来了解肥胖在马的免疫系统中的作用，及其在内分泌紊乱发生发展中的作用。
