Bai Jiaxiang, Wang Huaiyu, Chen Hao, Ge Gaoran, Wang Miao, Gao Ang, Tong Liping, Xu Yaozeng, Yang Huiling, Pan Guoqing, Chu Paul K, Geng Dechun
Department of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou, Jiangsu, 215006, PR China.
Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, PR China; Department of Physics, Department of Materials Science & Engineering, and Department of Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, PR China.
Biomaterials. 2020 Oct;255:120197. doi: 10.1016/j.biomaterials.2020.120197. Epub 2020 Jun 13.
Bone endoprosthesis in patients with systemic chronic inflammation frequently leads to poor osseointegration after implantation mainly due to the increase in pro-inflammatory cytokines that induce bone resorption and impair bone formation. In this work, peptide-coated implants are designed to create a beneficial bone immune microenvironment around prostheses to promote interfacial osteogenesis. By taking advantage of the spontaneous and stable coordination chemistry, Ti-based implants are coated with the mussel-inspired peptide to mitigate lipopolysaccharide (LPS)-induced inflammation by up-regulating the M2 phenotype of macrophages. In addition, the peptide coating increases the bone-implant contact (BIC) by nearly 3 times resulting in suppressed osteoclastogenesis and promoted osteogenesis by inhibiting the nuclear factor kappa-B (NF-κB) signalling pathway. Our findings indicate that biomimetic peptides with osteoimmunomodulatory bioactivity can be incorporated into Ti-based prostheses to facilitate bone regeneration in patients with chronic inflammatory diseases.
对于患有全身性慢性炎症的患者，骨内植入物在植入后常常导致骨整合不良，这主要是由于促炎细胞因子增加，这些因子会诱导骨吸收并损害骨形成。在这项研究中，设计了肽涂层植入物，以在假体周围创建有益的骨免疫微环境，从而促进界面骨生成。利用自发且稳定的配位化学，在钛基植入物上涂覆受贻贝启发的肽，通过上调巨噬细胞的M2表型来减轻脂多糖（LPS）诱导的炎症。此外，肽涂层使骨-植入物接触（BIC）增加了近3倍，通过抑制核因子κB（NF-κB）信号通路，抑制破骨细胞生成并促进骨生成。我们的研究结果表明，具有骨免疫调节生物活性的仿生肽可被纳入钛基假体，以促进慢性炎症性疾病患者的骨再生。
