Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine (TCM), Nanchang, China.
Laboratory Animal Research Center for Science and Technology, Jiangxi University of Traditional Chinese Medicine (TCM), Nanchang, China.
Front Cell Infect Microbiol. 2022 Jun 27;12:907813. doi: 10.3389/fcimb.2022.907813. eCollection 2022.
The rising incidence of ulcerative colitis has become a new challenge for public health. Oliv. leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects. However, the therapeutic effect of COG in ulcerative colitis (UC) has not been reported. Here, the experimental colitis was treated with dextran sodium sulfate (DSS) and COG. After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved. COG significantly reduced the levels of inflammatory cytokines in UC mice and and restored the secretion levels of IL-6 and IL-10 in the colon. Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG. Moreover, less Th17 and more Tregs were observed in the COG-treated groups. In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and and were obviously regulated at the genus level. In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
溃疡性结肠炎的发病率不断上升，已成为公共卫生领域的新挑战。乳香酸（COG）是一种用于治疗呼吸系统疾病的天然药物，具有出色的抗炎和抗氧化作用。然而，COG 在溃疡性结肠炎（UC）中的治疗效果尚未见报道。在这里，我们使用葡聚糖硫酸钠（DSS）和 COG 处理实验性结肠炎。用高（30 g/kg）、中（15 g/kg）和低（7.5 g/kg）剂量的 COG 连续治疗 11 天，可有效改善模型小鼠的体重、疾病活动指数（DAI）评分、结肠长度、结肠重量指数和病理评分。COG 可显著降低 UC 小鼠的炎症细胞因子水平，并恢复结肠中 IL-6 和 IL-10 的分泌水平。同时，与结肠炎小鼠相比，COG 处理组的 MDA、MPO、NO 和 eNOS 水平较低，GSH-Px 和 MAO 水平较高，表明 COG 减轻了结肠炎小鼠的氧化应激损伤。此外，COG 处理组中 Th17 细胞减少，Treg 细胞增加。此外，COG 改善了结肠炎小鼠结肠中肠道微生物群落的多样性和相对丰度，在属水平上明显调节了 和 。总之，COG 对 DSS 诱导的实验性结肠炎具有保护作用，主要通过抑制免疫炎症反应和氧化应激，以及调节 mTreg 细胞反应和肠道菌群组成来发挥作用。
