State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Medical Research Institute of First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China 830011.
Department of Spinal Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China 830001.
Dis Markers. 2020 May 20;2020:2509454. doi: 10.1155/2020/2509454. eCollection 2020.
Macrophages are important immune cells involved in () infection. To further investigate the degree of disease development in patients with spinal tuberculosis (TB), we conducted research on macrophage polarization.
Thirty-six patients with spinal TB and twenty-five healthy controls were enrolled in this study. The specific morphology of tuberculous granuloma in spinal tissue was observed by hematoxylin-eosin (H&E) staining. The presence and distribution of bacilli were observed by Ziehl-Neelsen (ZN) staining. Macrophage-specific molecule CD68 was detected by immunohistochemistry (IHC). M1 macrophages play a proinflammatory role, including the specific molecule nitric oxide synthase (iNOS) and the related cytokine tumor necrosis factor- (TNF-) and interferon- (IFN-). M2 macrophages exert anti-inflammatory effects, including the specific molecule CD163 and related cytokine interleukin-10 (IL-10). The above markers were all detected by quantitative real-time PCR (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and IHC.
Typical tuberculous granuloma was observed in the HE staining of patients with spinal TB. ZN staining showed positive expression of Ag85B around the caseous necrosis tissue and Langerhans multinucleated giant cells. At the same time, IHC results indicated that CD68, iNOS, CD163, IL-10, TNF-, and IFN- were expressed around the tuberculous granuloma, and their levels were obviously higher in close tissue than in the distant tissue. RT-PCR and ELISA results indicated that IL-10, TNF-, and IFN- levels of TB patients were also higher than those of the healthy controls.
The report here highlights that two types of macrophage polarization (M1 and M2) are present in the tissues and peripheral blood of patients with spinal TB. Macrophages also play proinflammatory and anti-inflammatory roles. Macrophage polarization is involved in spinal TB infection.
巨噬细胞是参与（）感染的重要免疫细胞。为了进一步研究脊柱结核（TB）患者疾病发展的程度，我们对巨噬细胞极化进行了研究。
本研究纳入了 36 例脊柱 TB 患者和 25 名健康对照者。通过苏木精-伊红（H&E）染色观察脊柱组织中结核肉芽肿的特殊形态。通过齐尔-尼尔森（ZN）染色观察杆菌的存在和分布。通过免疫组织化学（IHC）检测巨噬细胞特异性分子 CD68。M1 巨噬细胞发挥促炎作用，包括特异性分子一氧化氮合酶（iNOS）和相关细胞因子肿瘤坏死因子-（TNF-）和干扰素-（IFN-）。M2 巨噬细胞发挥抗炎作用，包括特异性分子 CD163 和相关细胞因子白细胞介素-10（IL-10）。上述标志物均通过实时定量 PCR（RT-PCR）、酶联免疫吸附试验（ELISA）和 IHC 进行检测。
脊柱 TB 患者的 HE 染色显示典型的结核肉芽肿。ZN 染色显示在干酪样坏死组织和朗格汉斯多核巨细胞周围 Ag85B 呈阳性表达。同时，IHC 结果表明 CD68、iNOS、CD163、IL-10、TNF-和 IFN-在结核肉芽肿周围表达，其在邻近组织中的表达明显高于远处组织。RT-PCR 和 ELISA 结果表明，TB 患者的 IL-10、TNF-和 IFN-水平也高于健康对照组。
本报告强调了两种类型的巨噬细胞极化（M1 和 M2）存在于脊柱 TB 患者的组织和外周血中。巨噬细胞也发挥促炎和抗炎作用。巨噬细胞极化参与了脊柱 TB 感染。
