Cao Ying, Li Wei, Chu Xin, Wu Ke, Liu Haizhou, Liu Di
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei, China.
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Sheng Wu Gong Cheng Xue Bao. 2020 May 25;36(5):811-819. doi: 10.13345/j.cjb.190368.
Sequencing technology has been greatly improved in terms of throughput and cost. The single-molecule nanopore DNA sequencing, one of the major branches of the third-generation sequencing technology, has made great contributions in the fields of medicine and life sciences due to its advantages of ultra-long reading length, real-time detection and direct detection of base methylation modification, etc. This article briefly describes the principle of nanopore sequencing technology, and discusses its application in clinical, animal, plant, bacterial and virus fields and its future development direction.
测序技术在通量和成本方面有了很大的改进。单分子纳米孔DNA测序作为第三代测序技术的主要分支之一，因其超长读长、实时检测以及直接检测碱基甲基化修饰等优点，在医学和生命科学领域做出了巨大贡献。本文简要介绍了纳米孔测序技术的原理，并探讨了其在临床、动物、植物、细菌和病毒领域的应用及其未来发展方向。
