Li Zhonghong, DU Caili, Lin Yanfeng, Zhang Lieyu, Li Xiaoguang, Li Jiaxi, Chen Suhua
State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
School of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, Jiangxi, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Jan 25;38(1):5-13. doi: 10.13345/j.cjb.210085.
The development of high-throughput sequencing techniques enabled a deeper and more comprehensive understanding of environmental microbiology. Specifically, the third-generation sequencing techniques represented by nanopore sequencing have greatly promoted the development of environmental microbiology research due to its advantages such as long sequencing reads, fast sequencing speed, real-time monitoring of sequencing data, and convenient machine carrying, as well as no GC bias and no PCR amplification requirement. This review briefly summarized the technical principle and characteristics of nanopore sequencing, followed by discussing the application of nanopore sequencing techniques in the amplicon sequencing, metagenome sequencing and whole genome sequencing of environmental microorganisms. The advantages and challenges of nanopore sequencing in the application of environmental microbiology research were also analyzed.
高通量测序技术的发展使人们能够更深入、更全面地了解环境微生物学。具体而言，以纳米孔测序为代表的第三代测序技术，凭借其测序读长较长、测序速度快、可实时监测测序数据、便于携带仪器以及无GC偏好性且无需PCR扩增等优势，极大地推动了环境微生物学研究的发展。本文简要总结了纳米孔测序的技术原理和特点，随后探讨了纳米孔测序技术在环境微生物扩增子测序、宏基因组测序和全基因组测序中的应用。还分析了纳米孔测序在环境微生物学研究应用中的优势与挑战。
