Sun Xue-Hui, Fan Xin, Hu Kai-Li, Hu Wen-Ting
Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Weifang Medical University, Weifang 261000, China.
Dept. of Stomatology, The Affiliated Hospital of Weifang Medical University, Weifang 261000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2020 Jun 1;38(3):245-249. doi: 10.7518/hxkq.2020.03.003.
This study aimed to investigate the molecular mechanism of RAB1A in the proliferation, invasion, and metastasis of human tongue squamous cell carcinoma.
Western blot was used to detect the expression of RAB1A protein in human normal tongue epithelial cells (Hacat) and tongue squamous cell carcinoma Tca8113. The changes in RAB1A after plasmid transfection were also studied. The Tca8113 cells were named SiRAB1A/Tca8113 after RAB1A plasmid transfection. The expression of the epithelial-mesenchymal transition (EMT)-related markers of SiRAB1A/Tca8113 cells was also detected. CCK-8 assay was used to detect the proliferation of SiRAB1A/Tca8113 cells. Transwell and wound healing assays were used to detect the invasive and metastatic abilities of SiRAB1A/Tca8113 cells, respectively.
Western blot results showed that the expression of RAB1A in tongue squamous cell carcinoma cells was significantly higher than that in Hacat. RAB1A decreased significantly after SiRAB1A plasmid transfection. CCK-8 proliferation assay showed that the proliferation of SiRAB1A/Tca8113 cells also decreased significantly. Transwell and wound healing assays demonstrated that the invasive and metastatic abilities of SiRAB1A/Tca8113 cells decreased significantly, respectively. In addition, Western blot results demonstrated that RAB1A deletion significantly increased the expression of E-cadherin and inhibited the expression of Vimentin.
RAB1A could promote the proliferation, invasion, and metastasis of tongue squamous cell carcinoma cells.
本研究旨在探讨RAB1A在人舌鳞状细胞癌增殖、侵袭和转移中的分子机制。
采用蛋白质免疫印迹法检测RAB1A蛋白在人正常舌上皮细胞（Hacat）和舌鳞状细胞癌Tca8113中的表达。同时研究质粒转染后RAB1A的变化。RAB1A质粒转染后的Tca8113细胞命名为SiRAB1A/Tca8113。检测SiRAB1A/Tca8113细胞上皮-间质转化（EMT）相关标志物的表达。采用CCK-8法检测SiRAB1A/Tca8113细胞的增殖情况。分别采用Transwell法和划痕愈合实验检测SiRAB1A/Tca8113细胞的侵袭和转移能力。
蛋白质免疫印迹结果显示，RAB1A在舌鳞状细胞癌细胞中的表达明显高于Hacat细胞。SiRAB1A质粒转染后RAB1A显著降低。CCK-8增殖实验表明，SiRAB1A/Tca8113细胞的增殖也显著降低。Transwell法和划痕愈合实验表明，SiRAB1A/Tca8113细胞的侵袭和转移能力分别显著降低。此外，蛋白质免疫印迹结果表明，RAB1A缺失显著增加E-钙黏蛋白的表达并抑制波形蛋白的表达。
RAB1A可促进舌鳞状细胞癌细胞的增殖、侵袭和转移。
