Seow W K, Thong Y H, McCormack J G, Ferrante A
Department of Child Health, University of Queensland, South Brisbane, Australia.
Int Arch Allergy Appl Immunol. 1988;85(1):63-8. doi: 10.1159/000234475.
Human neutrophil adherence was enhanced by recombinant human tumour necrosis factor-beta (TNF beta) but suppressed by recombinant human interleukin-2 (IL-2). The opposite effects of these two lymphokines were observed over a range of concentrations consistent with their other biological activities, occurred within 15 min of incubation, and were still evident after 60 min. Pretreatment of neutrophils with both IL-2 and TNF beta resulted in adherence values intermediate between the values obtained with the individual lymphokines. IL-2 suppressed the stimulatory effects of both the chemotactic peptide formyl-methionyl-leucyl-phenyl-alanine (FMLP) and the phorbol ester phorbol myristate acetate (PMA). The combination of TNF beta with either FMLP or PMA produced enhancement of neutrophil adherence which exceeded that of either agent alone. These effects of the lymphokines were not due to endotoxin contamination since their effects were sensitive to heating and insensitive to polymyxin B treatment. These experiments provide further evidence for the critical role of these lymphokines in the regulation of acute and chronic inflammatory processes.
重组人肿瘤坏死因子-β（TNF-β）可增强人中性粒细胞的黏附性，但重组人白细胞介素-2（IL-2）则抑制其黏附性。在与其其他生物学活性相符的一系列浓度范围内，观察到这两种淋巴因子的相反作用，在孵育15分钟内出现，且在60分钟后仍很明显。用IL-2和TNF-β对中性粒细胞进行预处理后，黏附值介于单独使用这两种淋巴因子时获得的值之间。IL-2抑制趋化肽甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）和佛波酯佛波醇肉豆蔻酸酯乙酸酯（PMA）的刺激作用。TNF-β与FMLP或PMA联合使用可增强中性粒细胞的黏附性，其增强程度超过单独使用任何一种试剂。这些淋巴因子的这些作用并非内毒素污染所致，因为它们的作用对加热敏感且对多黏菌素B处理不敏感。这些实验为这些淋巴因子在急性和慢性炎症过程调节中的关键作用提供了进一步证据。
