Seow W K, Thong Y H, Ferrante A
Department of Child Health, University of Queensland, Mater Children's Hospital, South Brisbane, Australia.
Immunology. 1987 Nov;62(3):357-61.
Incubation of human neutrophils with recombinant human interleukin-1 alpha (IL-1 alpha) resulted in the suppression of neutrophil adherence. In contrast, similar treatment with recombinant human tumour necrosis factor-alpha (TNF alpha, cachectin) resulted in the enhancement of neutrophil adherence. These contrasting effects were noted as early as 5 min after incubation, and persisted for at least 60 min. Simultaneous addition of these two monokines resulted in intermediate values between suppression by IL-1 and enhancement by TNF alpha. The stimulatory effects of the chemotactic peptide FMLP and the phorbol ester PMA were ameliorated by IL-1 but augmented by TNF alpha. The effects of these monokines on neutrophil adherence were abolished by heating but not by polymyxin B treatment, showing that their modulatory properties were not mediated by endotoxin.
将人中性粒细胞与重组人白细胞介素-1α（IL-1α）一起孵育会导致中性粒细胞黏附受到抑制。相反，用重组人肿瘤坏死因子-α（TNFα，恶病质素）进行类似处理则会导致中性粒细胞黏附增强。早在孵育5分钟后就观察到了这些相反的效应，并且持续至少60分钟。同时添加这两种单核因子会产生介于IL-1抑制和TNFα增强之间的中间值。趋化肽FMLP和佛波酯PMA的刺激作用被IL-1减弱，但被TNFα增强。这些单核因子对中性粒细胞黏附的作用通过加热被消除，但多黏菌素B处理不能消除，这表明它们的调节特性不是由内毒素介导的。
