Laboratory of Biochemistry, Research Unit: UR 12ES08 "Cell Signaling and Pathologies", Faculté de Médecine de Monastir, University of Monastir, 5019, Monastir, Tunisia.
Laboratory of Animal Eco-physiology, Faculty of Sciences, Sfax University, Sfax, Tunisia.
BMC Pharmacol Toxicol. 2020 Jun 23;21(1):46. doi: 10.1186/s40360-020-00426-1.
Flavonoids, which existed nearly in all fruits and vegetables, are considered as a class of plant-secondary metabolites with a polyphenolic structure and have properties with health-improving potential. Yet, not so many experimental focus on the benefits of flavonoid in vivo after external application. Here we assessed the impacts of naringin in vitro and in vivo in the human glioma U-87 cells implanted into athymic mice.
Tumor size and animal survival time were followed in naringin-treated mice bearing subcutaneous gliomas. To define the effects of naringin on angiogenesis, in vitro, tube formation and migration were assayed using endothelial HUVEC cell line.
Low concentration of naringin remarkably inhibited tubulogenesis and reduced cell invasion. Moreover, naringin has been shown to have a toxicity effect on U-87 cells in a dose-dependent way. Furthermore, naringin administration (120 mg/kg/day) applies serious anti-cancer belongings on glioblastoma, as demonstrated by a slow cancer progression.
Our study has provided the first evidence on the antitumor effect of naringin, which is somehow due to the inhibition of invasion and angiogenesis.
黄酮类化合物几乎存在于所有的水果和蔬菜中，被认为是一类具有多酚结构和潜在健康促进特性的植物次生代谢产物。然而，体外应用黄酮类化合物后，体内的益处并没有得到太多的实验关注。在这里，我们评估了柚皮苷在体外和体内对植入无胸腺小鼠的人神经胶质瘤 U-87 细胞的影响。
在皮下种植神经胶质瘤的柚皮苷处理的小鼠中，监测肿瘤大小和动物存活时间。为了确定柚皮苷对血管生成的影响，我们使用内皮 HUVEC 细胞系在体外检测了管形成和迁移。
低浓度的柚皮苷显著抑制了管形成并减少了细胞侵袭。此外，柚皮苷对 U-87 细胞具有剂量依赖性的毒性作用。此外，柚皮苷（120mg/kg/天）给药对神经胶质瘤具有严重的抗癌作用，这表现在癌症进展缓慢。
我们的研究首次提供了柚皮苷的抗肿瘤作用的证据，这在某种程度上是由于抑制了侵袭和血管生成。
