Orson F M, Auzenne C A
Department of Internal Medicine, Baylor College of Medicine, Houston, Texas.
Cell Immunol. 1988 Mar;112(1):147-55. doi: 10.1016/0008-8749(88)90283-3.
Glucocorticosteroid (GCS)-induced immunoglobulin (Ig) production in vitro is dependent on the functions of T cells and monocytes. T cells produce a replacing factor (TRF-S) which, with monocytes and a broad spectrum of concentrations (both above and below the physiologic range) of GCS, stimulates B cells to synthesize Ig. TRF-S is produced by T cells in cultures of mononuclear cells in the absence of stimulation over the initial 72 hr in culture. T cells, however, require the presence of monocytes or small quantities of interleukin 1 in order for the synthesis of TRF-S to occur. In addition to their role in stimulating TRF-S production, monocytes are also required in cultures of B cells responding to GCS and the cytokine. These experiments demonstrate that this monocyte function cannot be replaced by IL-1 or crude supernatants of monocyte cultures. Furthermore, exposure of TRF-S containing supernatants to oxidizing conditions does not alter the dependence of the cytokine on monocytes or GCS. Coculture of B cells and monocytes separated by a permeable membrane demonstrated that the influence of monocytes on GCS-induced Ig production is unlikely to be mediated by stable soluble factors. Thus, GCS-induced Ig production requires intimate contact between monocytes and B cells in the form of surface contact or unstable soluble mediators.
体外糖皮质激素（GCS）诱导的免疫球蛋白（Ig）产生依赖于T细胞和单核细胞的功能。T细胞产生一种替代因子（TRF-S），在单核细胞以及广泛浓度范围（生理范围上下）的GCS存在时，该因子刺激B细胞合成Ig。在最初培养的72小时内，在无刺激的单核细胞培养物中，T细胞可产生TRF-S。然而，T细胞需要单核细胞或少量白细胞介素1的存在才能发生TRF-S的合成。除了在刺激TRF-S产生中发挥作用外，在对GCS和细胞因子作出反应的B细胞培养中也需要单核细胞。这些实验表明，这种单核细胞功能不能被白细胞介素1或单核细胞培养物的粗提上清液所替代。此外，将含有TRF-S的上清液暴露于氧化条件下，不会改变该细胞因子对单核细胞或GCS的依赖性。用可渗透膜分隔B细胞和单核细胞进行共培养表明，单核细胞对GCS诱导的Ig产生的影响不太可能由稳定的可溶性因子介导。因此，GCS诱导的Ig产生需要单核细胞与B细胞以表面接触或不稳定的可溶性介质的形式进行密切接触。
