Born R, Trott K R
Institute of Radiobiology, GSF, Neuherberg, F.R. Germany.
Int J Radiat Biol Relat Stud Phys Chem Med. 1988 Feb;53(2):319-30. doi: 10.1080/09553008814550671.
The clonogenic potential of the progeny of irradiated cells was tested in vitro by replating irradiated cultures after various times, allowing between five and over 25 subsequent divisions to take place after irradiation. Whereas the plating efficiency of surviving Chinese hamster cells was not decreased, in C3H10T1/2 cells a dose-dependent but slight decrease in plating efficiency was observed even after the longest follow-up period. These data do not contradict the prevalent hypothesis in radiobiology that the proliferation potential of a clonogenic cell surviving after irradiation is not significantly different from that of a non-irradiated cell.
通过在不同时间重新接种受辐照培养物，使受辐照细胞的子代在体外进行克隆形成试验，在辐照后进行5至25次以上的后续分裂。虽然存活的中国仓鼠细胞的接种效率没有降低，但在C3H10T1/2细胞中，即使在最长的随访期后，也观察到接种效率呈剂量依赖性但略有下降。这些数据与放射生物学中普遍存在的假设并不矛盾，即辐照后存活的克隆形成细胞的增殖潜力与未受辐照细胞的增殖潜力没有显著差异。
