Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, PR China.
Shanghai municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, PR China.
Physiol Behav. 2020 Oct 1;224:113024. doi: 10.1016/j.physbeh.2020.113024. Epub 2020 Jun 21.
This study aimed to explore the neurobiological effects of "Chinese Traditional Five-Elements Music Therapy" on rats and to determine its effects on amino acid neurotransmitter levels, the excitatory/inhibitory(E/I) balance and the Glu-Gln cycle.
Male Wistar rats were assigned at random to the experimental groups (Gong/powerful; Shang/sad; Jue/gentle; Zhi/joyful; Yu/serene music group) and the control group(n=8/group).The experimental groups were exposed daily to music(2 hours per day; mild sound pressure levels, between 50 and 60 dB) for 28 consecutive days. Finally, we hypothesized concentrations of Glu and GABA to match the music types and measured additionally Asp, Gly, Gln, and Glu/GABA ratio in striatum by UPLC-3QMS.
Effects in the predicted direction were observed for Gong (Glu +, GABA -); Shang (Glu -; GABA +); Jue (Glu 0; GABA 0); and Zhi (Glu +; GABA -); but not Yu music (Glu + contrary to hypothesis; GABA + as hypothesized)." In addition, significant difference in Gln levels were also present in the zhi, Gong and Yu music groups compared to the controls.
Our study showed that different melodic music produced different effects on amino acid neurotransmitter levels. "Chinese Traditional Five-Elements Music Therapy" affected the amino acid neurotransmitter levels, the E/I balance and the Glu-Gln cycle in the striatum of rats, which may reflect altered glutamatergic and GABAergic system.
本研究旨在探讨“中医五行音乐疗法”对大鼠的神经生物学影响，并确定其对氨基酸神经递质水平、兴奋/抑制（E/I）平衡和 Glu-Gln 循环的影响。
雄性 Wistar 大鼠随机分为实验组（宫/有力；商/悲伤；角/柔和；徵/欢快；羽/平静音乐组）和对照组（n=8/组）。实验组每天接受音乐（每天 2 小时；轻度声压水平在 50 到 60 分贝之间）28 天。最后，我们假设谷氨酸和 GABA 的浓度与音乐类型相匹配，并通过 UPLC-3QMS 测量纹状体中的天冬氨酸、甘氨酸、谷氨酰胺和谷氨酸/ GABA 比值。
宫（Glu+，GABA-）；商（Glu-；GABA+）；角（Glu0；GABA0）；徵（Glu+；GABA-）表现出与预测方向一致的效果；但羽音乐（Glu+与假设相反；GABA+与假设相符）则不然。此外，与对照组相比，智、宫和羽音乐组的谷氨酰胺水平也存在显著差异。
本研究表明，不同旋律的音乐对氨基酸神经递质水平产生不同的影响。“中医五行音乐疗法”影响大鼠纹状体的氨基酸神经递质水平、E/I 平衡和 Glu-Gln 循环，这可能反映了谷氨酸能和 GABA 能系统的改变。
