长链非编码 RNA-ATB 减轻血管内皮细胞中血管紧张素 II 诱导的损伤。
Long Non-Coding RNA-ATB Attenuates the Angiotensin II-Induced Injury of Vascular Endothelial Cell.
机构信息
Deparment of Cardiovascular Medicine, Xian Ning Central Hospital, the First Affiliated Hospital of Hubei University of Science and Technology, Xian Ning Central Hospital, Xian'an District, Xian Ning City, Hubei Province, China.
Deparment of Geriatric, Xian Ning Central Hospital, the First Affiliated Hospital of Hubei University of Science and Technology, Xian Ning Central Hospital, Xian'an District, Xian Ning City, Hubei Province, China
出版信息
Ann Clin Lab Sci. 2020 May;50(3):378-382.
OBJECTIVE
Long noncoding RNA activated by transforming growth factor-II (lncRNA-ATB) has been reported to have critical roles in carcinogenesis and progression of several cancers. However, the expression and biological roles of lncRNA-ATB in cardiovascular disease are still unclear. This study aimed to investigate the role of lncRNA-ATB expression in endothelial cell injury.
MATERIALS
Angiotensin II (Ang II)-induced damage to endothelial cells (ECs) plays a crucial role in the research of cardiovascular disease; it is used to treat human umbilical vein endothelial cell (HUVECs) at different concentrations. qRT-PCR is used to identify the expression of lncRNA-ATB in HUVECs. CCK-8 assay and flow cytometry analysis are performed to assess cell viability and apoptosis of HUVECs.
RESULTS
Ang II treatment down-regulated the lncRNA-ATB level in HUVECs. Meanwhile, both knockdown of lncRNA-ATB and Ang II treatment impaired the viability, and increased apoptosis rate of cells. Furthermore, overexpression of lncRNA-ATB inhibited cell apoptosis and repaired cell viability in HUVECs.
CONCLUSION
The findings indicated that lncRNA-ATB might be associated with the development of the cardiovascular disease.
目的
转化生长因子-II 激活的长非编码 RNA（lncRNA-ATB）已被报道在几种癌症的发生和进展中具有关键作用。然而，lncRNA-ATB 在心血管疾病中的表达和生物学作用尚不清楚。本研究旨在探讨 lncRNA-ATB 表达在血管内皮细胞损伤中的作用。
材料
血管紧张素 II（Ang II）诱导的内皮细胞（ECs）损伤在心血管疾病的研究中起着至关重要的作用；它被用于以不同浓度处理人脐静脉内皮细胞（HUVECs）。qRT-PCR 用于鉴定 HUVECs 中 lncRNA-ATB 的表达。CCK-8 测定和流式细胞术分析用于评估 HUVECs 的细胞活力和凋亡。
结果
Ang II 处理下调了 HUVECs 中的 lncRNA-ATB 水平。同时，lncRNA-ATB 的敲低和 Ang II 处理均损害了细胞的活力，并增加了细胞的凋亡率。此外，lncRNA-ATB 的过表达抑制了 HUVECs 中的细胞凋亡并修复了细胞活力。
结论
这些发现表明，lncRNA-ATB 可能与心血管疾病的发展有关。
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