Saffarzadeh Negin, Ghafouri-Fard Soudeh, Rezaei Zahra, Aghazadeh Keyvan, Yazdani Farzad, Mohebi Mehdi, Ahmadi Mohsen, Shakoori Abbas, Tavakkoly-Bazzaz Javad
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cancer Manag Res. 2020 Jun 2;12:4085-4096. doi: 10.2147/CMAR.S252962. eCollection 2020.
Head and neck squamous cell carcinoma (HNSCC) includes a group of heterogeneous tumors with generally invasive behavior. The PI3K/AKT pathway plays an important role in the pathogenesis of HNSCC.
In the current study, we investigated the expression of two negative feedback regulators of the PI3K pathway, namely and , in 45 paired samples of HNSCC and adjacent non-cancerous tissues (ANCTs).
While expression of was down-regulated in tumoral tissues compared with ANCTs by the factor 4.21, expression levels were up-regulated by 5.99-times. Gender-based analysis revealed a significant down-regulation of gene expression level in male patients compared with the control samples and significant up-regulation of gene expression level in both sexes compared with the control samples. Differences in the expressions of both genes were significant in patients aged more than 60 years, but not in the younger patients. Expression of was only down-regulated in patients with positive smoking history. Expression of was decreased in grades 2 and 3 samples compared with controls. There was a significant increase in transcript levels of in stages II and III HNSCC samples compared with the controls group. ROC curve analysis indicated that the expression level of could be a promising marker for the diagnosis of HNSCC patients with a sensitivity and specificity of 0.666 and 0.688, respectively. In addition, sensitivity and specificity of were 0.755 and 0.577, respectively.
The current study indicates dysregulation of regulators of PI3K pathway in HNSCC and their potential application as putative biomarkers for this cancer.
头颈部鳞状细胞癌（HNSCC）包括一组具有普遍侵袭行为的异质性肿瘤。PI3K/AKT通路在HNSCC的发病机制中起重要作用。
在本研究中，我们调查了PI3K通路的两个负反馈调节因子，即[此处原文缺失两个调节因子的具体名称]，在45对HNSCC及其相邻非癌组织（ANCTs）样本中的表达情况。
与ANCTs相比，肿瘤组织中[第一个调节因子名称]的表达下调了4.21倍，而[第二个调节因子名称]的表达水平上调了5.99倍。基于性别的分析显示，与对照样本相比，男性患者中[第一个调节因子名称]基因表达水平显著下调，而与对照样本相比，两性中[第二个调节因子名称]基因表达水平均显著上调。这两个基因的表达差异在60岁以上的患者中显著，但在年轻患者中不显著。[第一个调节因子名称]的表达仅在有阳性吸烟史的患者中下调。与对照组相比，2级和3级样本中[第二个调节因子名称]的表达降低。与对照组相比，II期和III期HNSCC样本中[第一个调节因子名称]的转录水平显著增加。ROC曲线分析表明，[第一个调节因子名称]的表达水平可能是诊断HNSCC患者的一个有前景的标志物，其敏感性和特异性分别为0.666和0.688。此外，[第二个调节因子名称]的敏感性和特异性分别为0.755和0.577。
本研究表明HNSCC中PI3K通路调节因子的失调及其作为该癌症潜在生物标志物的应用潜力。
