Musungu Bryan, Bhatnagar Deepak, Quiniou Sylvie, Brown Robert L, Payne Gary A, O'Brian Greg, Fakhoury Ahmad M, Geisler Matt
Department of Plant Biology, Southern Illinois University, Carbondale, IL, United States.
Southern Regional Research Center, USDA-ARS, New Orleans, LA, United States.
Front Microbiol. 2020 Jun 3;11:853. doi: 10.3389/fmicb.2020.00853. eCollection 2020.
The interaction between and is complex, and the identification of plant genes and pathways conferring resistance to the fungus has been challenging. Therefore, the authors undertook a systems biology approach involving dual RNA-seq to determine the simultaneous response from the host and the pathogen. What was dramatically highlighted in the analysis is the uniformity in the development patterns of gene expression of the host and the pathogen during infection. This led to the development of a "stage of infection index" that was subsequently used to categorize the samples before down-stream system biology analysis. Additionally, we were able to ascertain that key maize genes in pathways such as the jasmonate, ethylene and ROS pathways, were up-regulated in the study. The stage of infection index used for the transcriptomic analysis revealed that produces a relatively limited number of transcripts during the early stages (0 to 12 h) of infection. At later stages, in , transcripts and pathways involved in endosomal transport, aflatoxin production, and carbohydrate metabolism were up-regulated. Multiple WRKY genes targeting the activation of the resistance pathways (i.e., jasmonate, phenylpropanoid, and ethylene) were detected using causal inference analysis. This analysis also revealed, for the first time, the activation of resistance genes influencing the expression of specific genes. Our results show that seems to be reacting to a hostile environment resulting from the activation of resistance pathways in . This study revealed the dynamic nature of the interaction between the two organisms.
[此处原文中两个空格处内容缺失，无法准确翻译完整句子]与[此处原文中两个空格处内容缺失，无法准确翻译完整句子]之间的相互作用很复杂，鉴定赋予植物对该真菌抗性的基因和途径具有挑战性。因此，作者采用了一种系统生物学方法，涉及双重RNA测序，以确定宿主和病原体的同步反应。分析中显著突出的是宿主和病原体在感染期间基因表达发育模式的一致性。这导致了“感染阶段指数”的开发，随后在下游系统生物学分析之前用于对样本进行分类。此外，我们能够确定在茉莉酸、乙烯和ROS等途径中的关键玉米基因在该研究中被上调。用于转录组分析的感染阶段指数显示，[此处原文中两个空格处内容缺失，无法准确翻译完整句子]在感染早期阶段（0至12小时）产生的转录本数量相对有限。在后期阶段，[此处原文中两个空格处内容缺失，无法准确翻译完整句子]中参与内体运输、黄曲霉毒素产生和碳水化合物代谢的转录本和途径被上调。使用因果推断分析检测到多个靶向抗性途径（即茉莉酸、苯丙烷类和乙烯）激活的WRKY基因。该分析还首次揭示了影响特定[此处原文中两个空格处内容缺失，无法准确翻译完整句子]基因表达的[此处原文中两个空格处内容缺失，无法准确翻译完整句子]抗性基因的激活。我们的结果表明，[此处原文中两个空格处内容缺失，无法准确翻译完整句子]似乎在对[此处原文中两个空格处内容缺失，无法准确翻译完整句子]中抗性途径激活所产生的不利环境做出反应。这项研究揭示了这两种生物体之间相互作用的动态性质。
