Yin Huadong, He Haorong, Cao Xinao, Shen Xiaoxu, Han Shunshun, Cui Can, Zhao Jing, Wei Yuanhang, Chen Yuqi, Xia Lu, Wang Yan, Li Diyan, Zhu Qing
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Front Genet. 2020 Jun 4;11:512. doi: 10.3389/fgene.2020.00512. eCollection 2020.
As bioinformatic approaches have been developed, it has been demonstrated that microRNAs (miRNAs) are involved in the formation of muscles and play important roles in regulation of muscle cell proliferation and differentiation. Previously, it has been demonstrated that miR-148a-3p is one of the most abundant miRNAs in chicken skeletal muscle. Here, we build on that work and demonstrate that miR-148a-3p is important in the control of differentiation of chicken skeletal muscle satellite cells (SMSCs). Elevated expression of miR-148a-3p significantly promoted differentiation and inhibited apoptosis of SMSCs but did not affect proliferation. Furthermore, it was observed that the mesenchyme homeobox 2 (Meox2) is a target gene of miR-148a-3p and that miR-148a-3p can down-regulate expression of Meox2, which promote differentiation of SMSCs and suppress apoptosis. Furthermore, miR-148a-3p overexpression encouraged activation of the PI3K/AKT signaling pathway, which could be recovered by overexpression of Meox2. Overall, these findings suggest that microRNA-148a-3p is a potent promoter of myogenesis via direct targeting of Meox2 and increase of the PI3K/AKT signaling pathway in chicken SMSCs.
随着生物信息学方法的发展，已证明微小RNA（miRNA）参与肌肉形成，并在肌肉细胞增殖和分化的调节中发挥重要作用。此前，已证明miR-148a-3p是鸡骨骼肌中最丰富的miRNA之一。在此，我们基于该研究进一步证明，miR-148a-3p在鸡骨骼肌卫星细胞（SMSC）分化的控制中起重要作用。miR-148a-3p表达升高显著促进SMSC的分化并抑制其凋亡，但不影响增殖。此外，观察到间充质同源盒2（Meox2）是miR-148a-3p的靶基因，miR-148a-3p可下调Meox2的表达，从而促进SMSC的分化并抑制凋亡。此外，miR-148a-3p过表达促进了PI3K/AKT信号通路的激活，而Meox2过表达可使其恢复。总体而言，这些发现表明，微小RNA-148a-3p通过直接靶向Meox2并增强鸡SMSC中的PI3K/AKT信号通路，是肌生成的有效促进因子。
