环状 RNA 谱分析鉴定出一种丰富的环状 RNA circTMTC1，它通过海绵吸附 miR-128-3p 抑制鸡骨骼肌卫星细胞分化。
Circular RNA profiling identified an abundant circular RNA circTMTC1 that inhibits chicken skeletal muscle satellite cell differentiation by sponging miR-128-3p.
机构信息
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
出版信息
Int J Biol Sci. 2019 Aug 19;15(10):2265-2281. doi: 10.7150/ijbs.36412. eCollection 2019.
Myogenesis involves a series of complex cellular and developmental processes regulated by many genes, transcription factors and non-coding RNAs. Recent studies have demonstrated the involvement of circular RNAs (circRNAs) in myogenesis. While previous studies have established a role for some circRNAs, the precise functions and mechanisms of circRNAs in skeletal muscle development are still not completely understood in chicken. To identify potential circRNAs during chicken embryonic skeletal muscle development, rRNA libraries sequencing was performed in breast muscles from 12 broilers and 12 layers at four different embryonic points, embryonic day 10 (E10), E13, E16 and E19. Through circRNA differential expression analysis and target miRNA prediction, the circTMTC1 was predicted to participate in the embryonic muscle formation by sponging miRNA, which were verified in vitro experiments. We identified 228 differentially expressed circRNAs between broilers and layers (fold change >2; p-value < 0.05), and 43 circRNAs were differentially expressed at multiple embryonic days. circTMTC1, a novel circRNA transcribed from the TMTC1 gene, was expressed significantly higher in layers than in broilers at E10, E13 and E16. Furthermore, circTMTC1 knockdown accelerated proliferation and differentiation in chicken skeletal muscle satellite cells (SMSCs), besides, circTMTC1-overexpressing cells showed opposite effects. circTMTC1 functioned as a miR-128-3p sponge at the differentiation stage of SMSCs, and circTMTC1 inhibited the expression of miR-128-3p. Furthermore, miR-128-3p promoted differentiation of chicken SMSCs, and circTMTC1 inhibited the promotion effect of miR-128-3p on chicken SMSC differentiation. Our study revealed that circRNAs are differentially expressed during chicken embryonic development between the two chicken models, and circTMTC1 inhibits chicken SMSC differentiation by sponging miR-128-3p.
肌发生涉及许多基因、转录因子和非编码 RNA 调控的一系列复杂的细胞和发育过程。最近的研究表明环状 RNA（circRNA）参与了肌发生。虽然以前的研究已经确定了一些 circRNA 的作用，但 circRNA 在鸡骨骼肌发育中的精确功能和机制仍不完全清楚。为了在鸡胚胎骨骼肌发育过程中鉴定潜在的 circRNA，在 12 只肉鸡和 12 只蛋鸡的胸肌中进行了 rRNA 文库测序，在四个不同的胚胎点，即胚胎第 10 天（E10）、E13、E16 和 E19 天。通过 circRNA 差异表达分析和靶 miRNA 预测，预测 circTMTC1 通过海绵 miRNA 参与胚胎肌肉形成，该预测在体外实验中得到了验证。我们在肉鸡和蛋鸡之间鉴定了 228 个差异表达的 circRNA（倍数变化>2；p 值<0.05），并且在多个胚胎日有 43 个 circRNA 差异表达。circTMTC1 是一种从 TMTC1 基因转录的新型 circRNA，在 E10、E13 和 E16 时在蛋鸡中的表达明显高于肉鸡。此外，circTMTC1 敲低加速了鸡骨骼肌卫星细胞（SMSCs）的增殖和分化，而过表达 circTMTC1 的细胞则表现出相反的效果。circTMTC1 在 SMSCs 的分化阶段作为 miR-128-3p 的海绵，并且 circTMTC1 抑制 miR-128-3p 的表达。此外，miR-128-3p 促进了鸡 SMSCs 的分化，而 circTMTC1 抑制了 miR-128-3p 对鸡 SMSC 分化的促进作用。我们的研究表明，circRNA 在两种鸡模型之间的鸡胚胎发育过程中差异表达，circTMTC1 通过海绵 miR-128-3p 抑制鸡 SMSC 分化。
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