Song In-Bong, Han Hye-Ju, Kwon Jungkee
Central Research and Development, Between Inc, Iksan, Republic of Korea.
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Jeonbuk, Iksan, 54596 Republic of Korea.
Food Sci Biotechnol. 2020 Feb 10;29(7):969-976. doi: 10.1007/s10068-020-00734-6. eCollection 2020 Jul.
Gamma radiation changes the molecular structure and activity of proteins, which in turn changes their physiological effects. Sericin, one of the silk peptides, has beneficial effects to humans such as inducing apoptosis, acting as an anti-oxidant. The effects of gamma irradiation on the physiological activity of fibroin have been studied, but its effects on sericin alone have not yet been established. In this study, we assessed the effects of gamma irradiation on sericin (I-sericin) in regard to its inflammatory effects in vitro and in vivo. Our results showed that I-sericin (5 kGy) significantly increased nitric oxide production, proliferation of immune cells, and effectively attenuated lipopolysaccharide (LPS)-induced inflammation. The mice were fed I-sericin for 4 weeks and treated with LPS; they exhibited significantly increased proliferation of lymphocytes, activation of NK cells and decreased secretion of inflammatory cytokines These results suggest gamma-irradiated I-sericin as a valuable functional food supplement by immune-enhancing and anti-inflammation effects.
伽马辐射会改变蛋白质的分子结构和活性，进而改变其生理效应。丝胶蛋白是丝肽之一，对人体有诱导细胞凋亡、充当抗氧化剂等有益作用。人们已经研究了伽马辐射对丝素蛋白生理活性的影响，但对其单独对丝胶蛋白的影响尚未明确。在本研究中，我们评估了伽马辐射对丝胶蛋白（I-丝胶蛋白）在体外和体内的炎症效应。我们的结果表明，I-丝胶蛋白（5千戈瑞）显著增加了一氧化氮的产生、免疫细胞的增殖，并有效减轻了脂多糖（LPS）诱导的炎症。给小鼠喂食I-丝胶蛋白4周后再用LPS处理；它们的淋巴细胞增殖显著增加，自然杀伤细胞活化，炎症细胞因子分泌减少。这些结果表明，经伽马辐射的I-丝胶蛋白具有免疫增强和抗炎作用，是一种有价值的功能性食品补充剂。
