• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在白亚口鱼（Catostomus commersoni）中存在两个后叶催产素基因，它们在蛋白质编码区域均无内含子。

Two isotocin genes are present in the white sucker Catostomus commersoni both lacking introns in their protein coding regions.

作者信息

Figueroa J, Morley S D, Heierhorst J, Krentler C, Lederis K, Richter D

机构信息

Institut für Zellbiochemie und Klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Hamburg, FRG.

出版信息

EMBO J. 1989 Oct;8(10):2873-7. doi: 10.1002/j.1460-2075.1989.tb08435.x.

DOI:10.1002/j.1460-2075.1989.tb08435.x
PMID:2583084
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC401345/
Abstract

Two genes each encoding a distinct precursor protein to the hormone isotocin and a neurophysin-related protein are present in the teleost fish Catostomus commersoni. These precursors are referred to as isotocin 1 and 2. As shown by the polymerase chain reaction technique, both genes lack introns in their protein-coding sequences. Both genes are transcribed giving rise to mRNAs of 920 (isotocin 1) and 1020 (isotocin 2) bases, respectively. Based on the nucleotide sequences, the predicted isotocin precursors contain, besides the hormone moiety, a neurophysin-like protein that, in contrast to its mammalian counterpart, is extended at its C-terminus by a peptide which includes a leucine-rich core segment. This segment shows similarities to the copeptin of the mammalian vasopressin precursor that is known to possess prolactin-releasing activity. The data imply that the mammalian copeptin sequence was initially part of a larger ancestral neurophysin molecule.

摘要

在硬骨鱼科鱼类康氏胭脂鱼中存在两个基因，每个基因编码一种独特的激素异催产素前体蛋白和一种与神经垂体素相关的蛋白。这些前体被称为异催产素1和2。如聚合酶链反应技术所示，这两个基因在其蛋白质编码序列中都没有内含子。两个基因都被转录，分别产生920个碱基（异催产素1）和1020个碱基（异催产素2）的mRNA。根据核苷酸序列，预测的异催产素前体除了激素部分外，还含有一种神经垂体素样蛋白，与其哺乳动物对应物不同的是，它在C端被一个包含富含亮氨酸核心片段的肽段所延伸。该片段与已知具有催乳素释放活性的哺乳动物血管加压素前体的 copeptin 相似。数据表明，哺乳动物的copeptin序列最初是一个更大的祖先神经垂体素分子的一部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/401345/5221bff2c5f8/emboj00134-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/401345/ef0463c22200/emboj00134-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/401345/a4508c4ca919/emboj00134-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/401345/5221bff2c5f8/emboj00134-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/401345/ef0463c22200/emboj00134-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/401345/a4508c4ca919/emboj00134-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/401345/5221bff2c5f8/emboj00134-0102-a.jpg

相似文献

1
Two isotocin genes are present in the white sucker Catostomus commersoni both lacking introns in their protein coding regions.在白亚口鱼（Catostomus commersoni）中存在两个后叶催产素基因，它们在蛋白质编码区域均无内含子。
EMBO J. 1989 Oct;8(10):2873-7. doi: 10.1002/j.1460-2075.1989.tb08435.x.
2
Vasotocin and isotocin precursors from the white sucker, Catostomus commersoni: cloning and sequence analysis of the cDNAs.来自白亚口鱼（Catostomus commersoni）的血管催产素和异催产素前体：cDNA的克隆与序列分析
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5242-6. doi: 10.1073/pnas.86.14.5242.
3
Vasotocin genes of the teleost fish Catostomus commersoni: gene structure, exon-intron boundary, and hormone precursor organization.美洲河鲶硬骨鱼的血管紧张素基因：基因结构、外显子-内含子边界及激素前体组织
Biochemistry. 1990 Mar 13;29(10):2506-11. doi: 10.1021/bi00462a011.
4
Sequence analysis of a cDNA encoding an isotocin precursor and localization of the corresponding mRNA in the brain of the cartilaginous fish Torpedo marmorata.编码异催产素前体的cDNA序列分析及相应mRNA在软骨鱼电鳐大脑中的定位。
Mol Mar Biol Biotechnol. 1995 Jun;4(2):179-84.
5
Cloning and sequence analyses of cDNAs encoding vasotocin and isotocin precursors of chum salmon, Oncorhynchus keta: evolutionary relationships of neurohypophysial hormone precursors.大麻哈鱼（Oncorhynchus keta）血管紧张素和异催产素前体编码cDNA的克隆及序列分析：神经垂体激素前体的进化关系
J Comp Physiol B. 1991;160(6):601-8. doi: 10.1007/BF00571256.
6
Evolution of the vasopressin/oxytocin superfamily: characterization of a cDNA encoding a vasopressin-related precursor, preproconopressin, from the mollusc Lymnaea stagnalis.加压素/催产素超家族的进化：从淡水螺（椎实螺）中克隆出一种与加压素相关的前体——前降钙素原的cDNA的特性分析
Proc Natl Acad Sci U S A. 1992 May 15;89(10):4593-7. doi: 10.1073/pnas.89.10.4593.
7
Structure and organization of the isotocin and vasotocin genes from teleosts.硬骨鱼中异催产素和加压催产素基因的结构与组织
Adv Exp Med Biol. 1995;395:629-38.
8
Australian lungfish neurohypophysial hormone genes encode vasotocin and [Phe2]mesotocin precursors homologous to tetrapod-type precursors.澳大利亚肺鱼神经垂体激素基因编码与四足动物型前体同源的加压催产素和[苯丙氨酸2]中催产素前体。
Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13339-44. doi: 10.1073/pnas.94.24.13339.
9
Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA.从牛催产素-神经垂体素I前体cDNA推导的氨基酸序列。
Nature. 1983;302(5906):342-4. doi: 10.1038/302342a0.
10
Cloning and sequence analyses of vasotocin and isotocin precursor cDNAs in the masu salmon, Oncorhynchus masou: evolution of neurohypophysial hormone precursors.马苏大马哈鱼（Oncorhynchus masou）中加压催产素和异催产素前体cDNA的克隆与序列分析：神经垂体激素前体的进化
Zoolog Sci. 1992 Feb;9(1):157-67.

引用本文的文献

1
Urotensin I-CRF-Urocortins: a mermaid's tail.尾加压素I-促肾上腺皮质激素释放因子-尿皮质素：一条美人鱼的尾巴。
Gen Comp Endocrinol. 2009 Oct;164(1):7-14. doi: 10.1016/j.ygcen.2009.04.024. Epub 2009 May 4.
2
Structure-function relationships of the vasopressin prohormone domains.血管升压素原激素结构域的结构-功能关系
Cell Mol Neurobiol. 1998 Apr;18(2):173-91. doi: 10.1023/a:1022564803093.
3
Transgenic rats reveal functional conservation of regulatory controls between the Fugu isotocin and rat oxytocin genes.转基因大鼠揭示了河豚异催产素基因与大鼠催产素基因之间调控控制的功能保守性。

本文引用的文献

1
Rapid similarity searches of nucleic acid and protein data banks.核酸和蛋白质数据库的快速相似性搜索。
Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30. doi: 10.1073/pnas.80.3.726.
2
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。附录
Anal Biochem. 1984 Feb;137(1):266-7. doi: 10.1016/0003-2697(84)90381-6.
3
Hybridization of denatured RNA transferred or dotted nitrocellulose paper.转移或点样在硝酸纤维素膜上的变性RNA的杂交。
Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12462-6. doi: 10.1073/pnas.94.23.12462.
4
Special evolution of neurohypophysial hormones in cartilaginous fishes: asvatocin and phasvatocin, two oxytocin-like peptides isolated from the spotted dogfish (Scyliorhinus caniculus).软骨鱼类神经垂体激素的特殊进化：阿斯瓦托辛和法斯瓦托辛，从斑点角鲨（Scyliorhinus caniculus）中分离出的两种催产素样肽。
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11266-70. doi: 10.1073/pnas.91.23.11266.
5
Cloning and sequence analyses of cDNAs encoding vasotocin and isotocin precursors of chum salmon, Oncorhynchus keta: evolutionary relationships of neurohypophysial hormone precursors.大麻哈鱼（Oncorhynchus keta）血管紧张素和异催产素前体编码cDNA的克隆及序列分析：神经垂体激素前体的进化关系
J Comp Physiol B. 1991;160(6):601-8. doi: 10.1007/BF00571256.
6
Evolution of the vasopressin/oxytocin superfamily: characterization of a cDNA encoding a vasopressin-related precursor, preproconopressin, from the mollusc Lymnaea stagnalis.加压素/催产素超家族的进化：从淡水螺（椎实螺）中克隆出一种与加压素相关的前体——前降钙素原的cDNA的特性分析
Proc Natl Acad Sci U S A. 1992 May 15;89(10):4593-7. doi: 10.1073/pnas.89.10.4593.
Methods Enzymol. 1983;100:255-66. doi: 10.1016/0076-6879(83)00060-9.
4
Pituitary enzyme conversion of putative synthetic oxytocin precursor intermediates.
J Biol Chem. 1985 Aug 25;260(18):10118-24.
5
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.超螺旋测序：一种用于质粒DNA测序的快速简便方法。
DNA. 1985 Apr;4(2):165-70. doi: 10.1089/dna.1985.4.165.
6
Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.在氯化铵中与碱基组成无关的杂交：一种用于高度复杂基因文库寡核苷酸筛选的方法。
Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585-8. doi: 10.1073/pnas.82.6.1585.
7
An amphibian two-domain 'big' neurophysin: conformational homology with the mammalian MSEL-neurophysin/copeptin intermediate precursor shown by trypsin-sepharose proteolysis.一种两栖动物双结构域“大”神经垂体激素运载蛋白：经胰蛋白酶-琼脂糖凝胶蛋白水解显示与哺乳动物MSEL-神经垂体激素运载蛋白/ copeptin中间前体的构象同源性。
FEBS Lett. 1988 Mar 28;230(1-2):77-80. doi: 10.1016/0014-5793(88)80645-8.
8
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.通过聚合酶链反应生成单链DNA及其在HLA - DQA基因座直接测序中的应用。
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6. doi: 10.1073/pnas.85.20.7652.
9
Structure, processing and evolution of the neurohypophysial hormone-neurophysin precursors.神经垂体激素-神经垂体素前体的结构、加工与进化
Biochimie. 1988 Sep;70(9):1197-207. doi: 10.1016/0300-9084(88)90185-x.
10
The glycopeptide moiety of vasopressin-neurophysin precursor is neurohypophysial prolactin releasing factor.血管加压素-神经垂体素前体的糖肽部分是神经垂体催乳素释放因子。
Biochem Biophys Res Commun. 1988 Feb 29;151(1):524-9. doi: 10.1016/0006-291x(88)90625-0.