Zhou Xiaoqing, Huang Shengyun, Jiang Licheng, Zhang Shizhou, Li Wengang, Chen Zhanwei, Zhang Dongsheng
Department of Oral and Maxillofacial Surgery, First People's Hospital of Jining, Jining, Shandong 272111, P.R. China.
Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Oncol Lett. 2014 May;7(5):1549-1555. doi: 10.3892/ol.2014.1906. Epub 2014 Feb 21.
Reversion-inducing cysteine-rich protein with Kazal motifs (RECK), a glycosylphosphatidylinositol-anchored glycoprotein, inhibits the enzymatic activities of certain matrix metalloproteinases (MMPs). RECK has been studied in numerous human tumors, but the expression of RECK in salivary adenoid cystic carcinoma (SACC), and its correlation with patient prognosis, has never been investigated thus far. In the present study, the expression of RECK and MMP-2 was evaluated in two ACC cell lines and in 83 patients with SACC. The results of quantitative polymerase chain reaction and western blot analysis revealed that the ACC-2 and ACC-M cell lines expressed RECK and MMP-2 mRNA and protein. The immunohistochemical staining in the patients demonstrated that positive expression of RECK and MMP-2 was observed in 21/83 (25.3%) and 69/83 (83.1%) cases, respectively, and that RECK expression was significantly associated with the tumor-node-metastasis stage, histological grade and perineural invasion of patients with SACC (P<0.05). Furthermore, there was a significant association between the positive expression of RECK and that of MMP-2 (P<0.0001). Univariate and multivariate analyses confirmed that a lack of RECK expression was an independent and significant factor for the prediction of a poor prognosis. In conclusion, RECK is a promising prognostic marker and potential therapeutic agent in SACC.
带有Kazal基序的富含半胱氨酸的逆转诱导蛋白（RECK）是一种糖基磷脂酰肌醇锚定糖蛋白，可抑制某些基质金属蛋白酶（MMP）的酶活性。RECK已在多种人类肿瘤中得到研究，但迄今为止，RECK在涎腺腺样囊性癌（SACC）中的表达及其与患者预后的相关性尚未得到研究。在本研究中，评估了RECK和MMP-2在两种ACC细胞系以及83例SACC患者中的表达。定量聚合酶链反应和蛋白质印迹分析结果显示，ACC-2和ACC-M细胞系表达RECK和MMP-2的mRNA及蛋白。患者的免疫组织化学染色显示，RECK和MMP-2的阳性表达分别在21/83（25.3%）和69/83（83.1%）例中观察到，并且RECK表达与SACC患者的肿瘤-淋巴结-转移分期、组织学分级和神经周侵犯显著相关（P<0.05）。此外，RECK的阳性表达与MMP-2的阳性表达之间存在显著关联（P<0.0001）。单因素和多因素分析证实，RECK表达缺失是预测预后不良的一个独立且显著的因素。总之，RECK是SACC中有前景的预后标志物和潜在治疗药物。
