Enk C, Mosbech H
Department of Infectious Diseases, State University Hospital, Copenhagen, Denmark.
Int Arch Allergy Appl Immunol. 1988;85(3):308-11. doi: 10.1159/000234522.
Monocytes from 6 patients with asthma and positive bronchial challenge with extract from the house dust mite Dermatophagoides pteronyssinus (Dp) were stimulated with lipopolysaccharide (LPS) and allergen extract from Dp. In order to neutralize putative endotoxin contamination of the allergen extract, some cultures were incubated in the presence of polymyxin B. Interleukin-1 (IL-1) activity was assayed by the comitogenic activity of the crude monocyte supernatants on phytohemagglutinin-stimulated murine thymocytes. Our results suggest that, upon stimulation with LPS and allergen, monocytes from atopic patients possess a normal capacity to produce IL-1. Like monocytes from healthy controls, patient monocytes do not produce IL-1 spontaneously. The specificity of the allergen stimulus is not well-defined, and further characterization of the IL-1-inducing property awaits the availability of endotoxin-free allergen preparations.
对6例哮喘患者且支气管对屋尘螨提取物（粉尘螨）激发试验呈阳性的单核细胞，用脂多糖（LPS）和粉尘螨变应原提取物进行刺激。为了中和变应原提取物中可能存在的内毒素污染，一些培养物在多粘菌素B存在的情况下进行孵育。通过粗单核细胞上清液对植物血凝素刺激的小鼠胸腺细胞的促有丝分裂活性来测定白细胞介素-1（IL-1）活性。我们的结果表明，在LPS和变应原刺激下，特应性患者的单核细胞具有产生IL-1的正常能力。与健康对照者的单核细胞一样，患者的单核细胞不会自发产生IL-1。变应原刺激的特异性尚不清楚，IL-1诱导特性的进一步表征有待无内毒素变应原制剂的获得。
