Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.
Faculty of Arts and Science, Human Biology Program, University of Toronto, Toronto, Ontario M5S 1A1, Canada.
Mol Med Rep. 2020 Sep;22(3):1737-1746. doi: 10.3892/mmr.2020.11264. Epub 2020 Jun 22.
Circular RNAs (circRNAs) are a class of non‑coding RNAs that exhibit important regulatory roles in various biological processes. However, the role of circRNAs and their potential role in osteoblast differentiation and mineralization is unclear. The aim of the present study was to investigate the expression of mmu_circ_003795 and its effect on collagen type XV α 1 chain (COL15A1). First, it was identified that the expression levels of mmu_circ_003795 and osteopontin (OPN) were upregulated in the induced cells. Silencing of mmu_circ_003795 reduced the gene and protein levels of COL15A1 and OPN, whereas the expression level of mmu‑microRNA (miR)‑1249‑5p was upregulated. In addition, after 7 or 14 days of induction, alkaline phosphatase and Alizarin Red‑S staining were decreased in the mmu_circRNA_003795 inhibitory group compared with the negative control group. In conclusion, mmu_circ_003795 may regulate osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 cells via mmu‑miR‑1249‑5p by targeting COL15A1.
环状 RNA（circRNAs）是一类非编码 RNA，在各种生物过程中发挥着重要的调控作用。然而，circRNAs 的作用及其在成骨细胞分化和矿化中的潜在作用尚不清楚。本研究旨在探讨 mmu_circ_003795 的表达及其对胶原 XVα1 链（COL15A1）的影响。首先，鉴定出 mmu_circ_003795 和骨桥蛋白（OPN）的表达水平在诱导细胞中上调。沉默 mmu_circ_003795 降低了 COL15A1 和 OPN 的基因和蛋白水平，而 mmu-微小 RNA（miR）-1249-5p 的表达水平上调。此外，在诱导 7 或 14 天后，mmu_circRNA_003795 抑制组的碱性磷酸酶和茜素红 S 染色与阴性对照组相比减少。综上所述，mmu_circ_003795 可能通过靶向 COL15A1 调控 MC3T3-E1 和 MDPC23 细胞中的成骨细胞分化和矿化，通过 mmu-miR-1249-5p 发挥作用。
