CRISPR/dCas9 的各种递送方法的应用。

Application of Various Delivery Methods for CRISPR/dCas9.

机构信息

Department of Clinical Pharmacy, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, No. 55, Section 4, South Renmin Road, Chengdu, 610041, Sichuan, China.

Personalized Drug Therapy Key Laboratory of Sichuan Province, Chengdu, China.

出版信息

Mol Biotechnol. 2020 Aug;62(8):355-363. doi: 10.1007/s12033-020-00258-8. Epub 2020 Jun 24.

DOI:10.1007/s12033-020-00258-8

PMID:32583364

Abstract

As gene-editing technology has become more and more popular in the life sciences, CRISPR has brought good news to scientific researchers because of its efficiency, convenience, and wide application. Its wide application has also promoted the development of basic scientific research, agriculture, basic medicine, and clinical treatment. However, how the CRISPR/dCas9 system is effectively delivered to the target organs or cells is still unknown. This paper briefly introduces the CRISPR/dCas9 system and then lists some common delivery methods and their characteristics.

摘要

随着基因编辑技术在生命科学领域越来越受欢迎，CRISPR 因其效率高、使用方便、应用广泛，给科研人员带来了好消息。其广泛应用也推动了基础科学研究、农业、基础医学和临床治疗的发展。然而，CRISPR/dCas9 系统如何有效地递送到靶器官或细胞仍然未知。本文简要介绍了 CRISPR/dCas9 系统，然后列出了一些常见的递送方法及其特点。