Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, 567 Baekje-daero, deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, South Korea.
Mol Biotechnol. 2020 Aug;62(8):380-386. doi: 10.1007/s12033-020-00256-w. Epub 2020 Jun 24.
2-Nonenal is a long-chain aliphatic aldehyde containing nine carbons and an unsaturated bond. 2-Nonenal is the primary cause of odor associated with aging, with an unpleasant greasy and grassy odor. Lysosome, mitochondria, and peroxisome are significant organelles in eukaryotic cells that contain various hydrolases that degrade biomolecules. Proteins in mitochondria and peroxisome also contain aldehyde dehydrogenase. We performed trans-2-nonenal treatment using lysosomal-related enzymes extracted from hen egg white (HEW). As trans-2-nonenal is more structurally stable than cis-2-nonenal, it was selected as the target aldehyde. HEW contains various biologically active proteins and materials such as albumin, ovotransferrin, lysosome, peroxisome, and mitochondria. Here, complementary experiments were conducted to evaluate the role of lysosomal-related enzymes in the treatment of trans-2-nonenal. The activity of lysosomal-related enzymes was confirmed via antimicrobial test against E. coli. HPLC analysis was used to determine the reduction of trans-2-nonenal. The trans-2-nonenal treatment depended on the reaction time and enzyme concentration. Materials considered as an intermediate from trans-2-nonenal treatment were detected by GC/MS spectrometer. Under acidic conditions (pH 6), lysosomal-related enzymes were the most efficient in the treatment of trans-2-nonenal. Furthermore, based on differential pH testing, we found the conditions under which all the 50 ppm trans-2-nonenal was removed. Therefore, our results suggest that the lysosomal-related enzymes reduced trans-2-nonenal, suggesting clinical application as anti-aging deodorants.
2-壬烯醛是一种含有九个碳原子和一个不饱和键的长链脂肪醛。2-壬烯醛是与衰老相关的气味的主要原因，具有令人不快的油腻和草味。溶酶体、线粒体和过氧化物酶体是真核细胞中重要的细胞器，其中含有各种水解酶，可降解生物分子。线粒体和过氧化物酶体中的蛋白质也含有醛脱氢酶。我们使用从鸡蛋白（HEW）中提取的溶酶体相关酶进行反式-2-壬烯醛处理。由于反式-2-壬烯醛比顺式-2-壬烯醛结构更稳定，因此选择其作为目标醛。HEW 含有各种具有生物活性的蛋白质和物质，如白蛋白、卵转铁蛋白、溶酶体、过氧化物酶体和线粒体。在这里，进行了补充实验以评估溶酶体相关酶在反式-2-壬烯醛处理中的作用。通过对大肠杆菌的抗菌试验确认了溶酶体相关酶的活性。使用 HPLC 分析来确定反式-2-壬烯醛的减少量。反式-2-壬烯醛的处理取决于反应时间和酶浓度。通过 GC/MS 光谱仪检测到被认为是反式-2-壬烯醛处理中间产物的物质。在酸性条件（pH 6）下，溶酶体相关酶在处理反式-2-壬烯醛方面最为有效。此外，根据不同 pH 值的测试，我们找到了去除所有 50ppm 反式-2-壬烯醛的条件。因此，我们的结果表明，溶酶体相关酶降低了反式-2-壬烯醛，这表明它们可能作为抗衰老除臭剂在临床上得到应用。
