Zhengzhou Health Vocational College, Zhengzhou, Henan Province, People's Republic of China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820928508. doi: 10.1177/1533033820928508.
Many long noncoding RNAs reportedly have tumor suppressive roles or are oncogenic in esophageal cancer. We have previously performed a chip-based expression analysis of primary esophageal cancer tissues and found that the expression of LINC00634 in these tissues was higher than that in nontumor tissues. Quantitative real-time-polymerase chain reaction, cell counting kit-8, flow cytometry, caspase3/7 assay, dual-luciferase reporter assay, and restore assay were used to detect the proliferative and apoptotic effects of LINC00634 in esophageal cancer cells. The results showed that the expression of LINC00634 in these tissues was higher than that in nontumor tissues and associated with tumor-node-metastasis (TNM) stage of patients. Knockdown of LINC00634 decreased cell viability and increased cell apoptosis levels in EC9706 and EC1 cells. LINC00634 could target Bcl2L1 through miR-342-3p. In this study, we show that LINC00634 is upregulated in esophageal cancer. We also show that the knockdown of LINC00634 decreased cell viability and increased cell apoptosis levels in EC9706 and EC1 cells through the miR-342-3p/Bcl2L1 axis.
许多长链非编码 RNA 据报道在食管癌中具有肿瘤抑制作用或致癌作用。我们之前对原发性食管癌组织进行了基于芯片的表达分析，发现这些组织中 LINC00634 的表达高于非肿瘤组织。采用定量实时聚合酶链反应、细胞计数试剂盒-8、流式细胞术、caspase3/7 测定、双荧光素酶报告基因测定和恢复测定来检测 LINC00634 在食管癌细胞中的增殖和凋亡作用。结果表明，LINC00634 在这些组织中的表达高于非肿瘤组织，且与患者的肿瘤-淋巴结-转移（TNM）分期相关。LINC00634 的敲低降低了 EC9706 和 EC1 细胞的细胞活力并增加了细胞凋亡水平。LINC00634 可以通过 miR-342-3p 靶向 Bcl2L1。在这项研究中，我们表明 LINC00634 在食管癌中上调。我们还表明，通过 miR-342-3p/Bcl2L1 轴，LINC00634 的敲低降低了 EC9706 和 EC1 细胞的细胞活力并增加了细胞凋亡水平。
