Department of Pharmacy, Abdul Wali Khan University Mardan, 23200, Pakistan.
Department of Pharmacology, Institute of Basic Medical Sciences (IBMS), Khyber Medical University, Peshawar, Pakistan.
Cell Mol Biol (Noisy-le-grand). 2020 Jun 25;66(4):208-213.
Datura metel is traditionally used as a remedy for renal toxicity. However, the nephroprotection has not been scientifically validated yet. To evaluate the nephroprotective like effect of methanolic extract of D. metel in gentamicin induced mice model, mice of either sex were divided into groups. One group received normal saline as negative control. The 2nd group received gentamicin 100mg/kg for 8 days as positive control, 3rd group received 50mg/kg silymarin as standard, while the reaming groups received 100, 200 and 300 mg/kg of MEDM and gentamicin 100mg/kg, for 8 days. The blood and urine samples were collected on 9th day, animals were then dissected and whole kidneys were removed and preserved in formalin for later histological examinations. The level of serum creatinine, blood urea nitrogen, urine creatinine and urine urea were significantly (P<0.05) elevated and the renal MDA level was also elevated significantly (P<0.05) by gentamicin in mice. After the treatment of test animals with MEDM, the elevated level of serum and urine biomarkers by gentamicin were reversed by MEDM. The nephroprotective effect was found in dose dependent manner. As the MEDM significantly protected the nephrotoxicity via its antioxidant effect. The findings of our study thus proved the scientific background for the nephroprotective effect of MEDM.
曼陀罗传统上被用作治疗肾毒性的药物。然而，其肾保护作用尚未得到科学验证。为了评估曼陀罗甲醇提取物对庆大霉素诱导的小鼠模型的肾保护作用，将雌雄小鼠分为几组。一组接受生理盐水作为阴性对照。第二组接受庆大霉素 100mg/kg 连续 8 天作为阳性对照，第三组接受 50mg/kg 水飞蓟素作为标准，而其余组分别接受 100、200 和 300mg/kg 的 MEDM 和庆大霉素 100mg/kg，连续 8 天。第 9 天收集血液和尿液样本，然后解剖动物，取出整个肾脏并保存在福尔马林中，以备以后进行组织学检查。庆大霉素可显著升高血清肌酐、血尿素氮、尿肌酐和尿尿素（P<0.05），并显著升高肾 MDA 水平（P<0.05）。用 MEDM 治疗试验动物后，可逆转庆大霉素引起的血清和尿液生物标志物水平升高。这种肾保护作用呈剂量依赖性。由于 MEDM 通过其抗氧化作用显著保护了肾毒性。因此，我们的研究结果证明了 MEDM 具有肾保护作用的科学背景。
