Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City-700000, Vietnam.
Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell Mol Biol (Noisy-le-grand). 2020 Jun 25;66(4):264-269.
Phytol (PHY) is an acyclic natural diterpene alcohol and a chlorophyll constituent that exhibits several pharmacological effects, such as anticancer, antioxidant, and antimicrobial. Here, we aimed to assess the PHY anti-inflammatory effect in vitro and in vivo, and to deepen knowledge on the possible mechanism of action. For this purpose, egg albumin (in vitro) test was performed by using acetyl salicylic acid (ASA) as a standard nonsteroidal anti-inflammatory drugs (NSAID). For in vivo test, male Wistar albino rats were treated (intraperitoneally) with 100 mg/kg of PHY and/or standard NSAIDs ASA (100 mg/kg) and diclofenac sodium (Diclo-Na, 10 mg/kg) to evaluate the combined effect of PHY in formalin-induced paw edema model. Furthermore, an in silico (CADD) study was accomplished to assess the effect of PHY against cyclooxygenase (COX)-1 and 2 enzymes, nuclear factor kappa B (NF-κB), and interleukin-1β (IL-1β). Results revealed that PHY exhibits dose-dependent anti-inflammatory effect using the egg albumin method. PHY (100 mg/kg) co-treated with ASA and/or Diclo-Na reduced paw edema better than PHY alone or NSAIDs individual groups. Computational study showed that PHY efficiently interacts with COX-1 and 2, NF-κB, and IL-1β. In conclusion, PHY exhibits anti-inflammatory activity, possibly via COX-1 and 2, NF-κB, and IL-1β dependent pathways.
植物醇（PHY）是一种无环天然二萜醇，也是叶绿素的组成部分，具有多种药理作用，如抗癌、抗氧化和抗菌。在这里，我们旨在评估 PHY 在体外和体内的抗炎作用，并深入了解其可能的作用机制。为此，采用乙酰水杨酸（ASA）作为标准非甾体抗炎药（NSAID）进行卵白蛋白（体外）试验。在体内试验中，雄性 Wistar 白化大鼠用 100mg/kg 的 PHY 和/或标准 NSAIDs ASA（100mg/kg）和双氯芬酸钠（Diclo-Na，10mg/kg）腹腔内给药，以评估 PHY 在福马林诱导的爪肿胀模型中的联合作用。此外，还进行了计算机辅助药物设计（CADD）研究，以评估 PHY 对环氧化酶（COX）-1 和 2 酶、核因子 kappa B（NF-κB）和白细胞介素-1β（IL-1β）的作用。结果表明，PHY 在用卵白蛋白法进行的抗炎试验中呈现出剂量依赖性的抗炎作用。用 100mg/kg 的 PHY 与 ASA 和/或 Diclo-Na 联合治疗，比单独使用 PHY 或 NSAIDs 单个组减轻爪肿胀的效果更好。计算研究表明，PHY 能有效地与 COX-1 和 2、NF-κB 和 IL-1β相互作用。总之，PHY 表现出抗炎活性，可能通过 COX-1 和 2、NF-κB 和 IL-1β依赖的途径。
