Smythers Amanda L, McConnell Evan W, Lewis Hailey C, Mubarek Saher N, Hicks Leslie M
Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
Plants (Basel). 2020 Jun 23;9(6):784. doi: 10.3390/plants9060784.
As global temperatures climb to historic highs, the far-reaching effects of climate change have impacted agricultural nutrient availability. This has extended to low latitude oceans, where a deficit in both nitrogen and phosphorus stores has led to dramatic decreases in carbon sequestration in oceanic phytoplankton. Although , a freshwater model green alga, has shown drastic systems-level alterations following nitrogen deprivation, the mechanisms through which these alterations are triggered and regulated are not fully understood. This study examined the role of reversible oxidative signaling in the nitrogen stress response of . Using oxidized cysteine resin-assisted capture enrichment coupled with label-free quantitative proteomics, 7889 unique oxidized cysteine thiol identifiers were quantified, with 231 significantly changing peptides from 184 proteins following 2 h of nitrogen deprivation. These results demonstrate that the cellular response to nitrogen assimilation, photosynthesis, pigment biosynthesis, and lipid metabolism are regulated by reversible oxidation. An enhanced role of non-damaging oxidative pathways is observed throughout the photosynthetic apparatus that provides a framework for further analysis in phototrophs.
随着全球气温攀升至历史新高，气候变化的深远影响已波及农业养分的可利用性。这种影响已延伸至低纬度海洋，那里氮和磷储备的不足导致海洋浮游植物的碳固存大幅下降。尽管淡水模式绿藻在氮缺乏后已显示出剧烈的系统水平变化，但其引发和调控这些变化的机制尚未完全明晰。本研究探究了可逆氧化信号在该绿藻氮胁迫响应中的作用。利用氧化半胱氨酸树脂辅助捕获富集结合无标记定量蛋白质组学，对7889个独特的氧化半胱氨酸硫醇标识符进行了定量分析，在氮缺乏2小时后，有来自184种蛋白质的231个肽段发生了显著变化。这些结果表明，细胞对氮同化、光合作用、色素生物合成和脂质代谢的反应受可逆氧化调控。在整个光合机构中观察到非损伤性氧化途径的作用增强，这为光合生物的进一步分析提供了框架。
