Gu Kai, Liu Yuling, Jiang Ting, Cai Chuner, Zhao Hui, Liu Xuanhong, He Peimin
College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
Biology (Basel). 2022 Oct 25;11(11):1563. doi: 10.3390/biology11111563.
The main algal species of Ulva prolifera green tide in the coastal areas of China are four species, but after reaching the coast of Qingdao, U. prolifera becomes the dominant species, where the light intensity is one of the most important influencing factors. In order to explore the effects of short-term high light stress on the internal molecular level of cells and its coping mechanism, the transcriptome, proteome, metabolome, and lipid data of U. prolifera were collected. The algae were cultivated in high light environment conditions (400 μmol·m−2·s−1) for 12 h and measured, and the data with greater relative difference (p < 0.05) were selected, then analyzed with the KEGG pathway. The results showed that the high light stress inhibited the assimilation of U. prolifera, destroyed the cell structure, and arrested its growth and development. Cells entered the emergency defense state, the TCA cycle was weakened, and the energy consumption processes such as DNA activation, RNA transcription, protein synthesis and degradation, and lipid alienation were inhibited. A gradual increase in the proportion of the C4 pathway was recorded. This study showed that U. prolifera can reduce the reactive oxygen species produced by high light stress, inhibit respiration, and reduce the generation of NADPH. At the same time, the C3 pathway began to change to the C4 pathway which consumed more energy. Moreover, this research provides the basis for the study of algae coping with high light stress.
中国沿海地区浒苔绿潮的主要藻类物种有四种，但浒苔到达青岛海岸后成为优势种，其中光照强度是最重要的影响因素之一。为了探究短期高光胁迫对细胞内分子水平的影响及其应对机制，收集了浒苔的转录组、蛋白质组、代谢组和脂质数据。将藻类在高光环境条件（400 μmol·m−2·s−1）下培养12 h后进行测定，选取相对差异较大（p < 0.05）的数据，然后用KEGG通路进行分析。结果表明，高光胁迫抑制了浒苔的同化作用，破坏了细胞结构，使其生长发育停滞。细胞进入应急防御状态，三羧酸循环减弱，DNA激活、RNA转录、蛋白质合成与降解以及脂质异化等能量消耗过程受到抑制。记录到C4途径的比例逐渐增加。该研究表明，浒苔可以减少高光胁迫产生的活性氧，抑制呼吸作用，减少NADPH的生成。同时，C3途径开始向消耗更多能量的C4途径转变。此外，本研究为藻类应对高光胁迫的研究提供了依据。
