内皮源性舒张因子可减少血小板与牛内皮细胞的黏附。
Endothelium-derived relaxing factor reduces platelet adhesion to bovine endothelial cells.
作者信息
Sneddon J M, Vane J R
机构信息
William Harvey Research Institute, Saint Bartholomew's Hospital Medical College, London, United Kingdom.
出版信息
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2800-4. doi: 10.1073/pnas.85.8.2800.
Abstract
The adhesion of thrombin-stimulated human blood platelets to either the endothelial surface of intact bovine aorta or cultured bovine aortic endothelial cells was studied to determine the role of endothelium-derived relaxing factor in the regulation of platelet adhesion. Endothelial cells and platelets were pretreated with indomethacin to prevent the formation of prostaglandins. The adhesion of thrombin-stimulated platelets to endothelial cells was reduced by superoxide dismutase and bradykinin. The inhibitory effect of both drugs was abolished by hemoglobin and was absent in strips of bovine aorta where the endothelial cells had been removed by scraping. It is suggested that the effects of bradykinin are mediated by the release of endothelium-derived relaxing factor, which is protected from destruction by superoxide dismutase, and that endothelium-derived relaxing factor contributes to the nonadhesive properties of the vascular endothelium.
摘要
研究了凝血酶刺激的人血小板与完整牛主动脉内皮表面或培养的牛主动脉内皮细胞的黏附情况，以确定内皮衍生舒张因子在调节血小板黏附中的作用。内皮细胞和血小板用吲哚美辛预处理以防止前列腺素的形成。超氧化物歧化酶和缓激肽可降低凝血酶刺激的血小板与内皮细胞的黏附。两种药物的抑制作用均被血红蛋白消除，且在通过刮擦去除内皮细胞的牛主动脉条中不存在这种抑制作用。提示缓激肽的作用是由内皮衍生舒张因子的释放介导的，超氧化物歧化酶可保护其免受破坏，且内皮衍生舒张因子有助于血管内皮的非黏附特性。
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本文引用的文献
1
Platelet adhesion to cultured vascular endothelial cells. A quantitative monolayer adhesion assay.血小板与培养的血管内皮细胞的黏附。一种定量单层黏附测定法。
J Lab Clin Med. 1982 Sep;100(3):425-36.
2
Identification of an endothelial cell cofactor for thrombin-catalyzed activation of protein C.鉴定凝血酶催化蛋白C活化的内皮细胞辅因子。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2249-52. doi: 10.1073/pnas.78.4.2249.
3
The effect of aspirin inhibition of PGI2 production on platelet adherence to normal and damaged rabbit aortae.阿司匹林抑制前列环素（PGI2）生成对血小板黏附于正常及受损兔主动脉的影响。
Thromb Res. 1980;17(3-4):453-64. doi: 10.1016/0049-3848(80)90080-8.
4
Endothelium-dependent inhibitory effects of acetylcholine, adenosine triphosphate, thrombin and arachidonic acid in the canine femoral artery.乙酰胆碱、三磷酸腺苷、凝血酶和花生四烯酸对犬股动脉的内皮依赖性抑制作用。
J Pharmacol Exp Ther. 1982 Jul;222(1):166-73.
5
On the regulation and control of fibrinolysis. Edward Kowalski Memorial Lecture.论纤维蛋白溶解的调控。爱德华·科瓦尔斯基纪念讲座。
Thromb Haemost. 1980 Jun 18;43(2):77-89.
6
The nature of endothelium-derived vascular relaxant factor.内皮源性血管舒张因子的本质。
Nature. 1984;308(5960):645-7. doi: 10.1038/308645a0.
7
An improved method for washing of human platelets with prostacyclin.一种用前列环素洗涤人血小板的改进方法。
Thromb Res. 1983 May 15;30(4):383-9. doi: 10.1016/0049-3848(83)90230-x.
8
Association between cyclic GMP accumulation and acetylcholine-elicited relaxation of bovine intrapulmonary artery.环磷酸鸟苷积累与乙酰胆碱诱导的牛肺内动脉舒张之间的关联。
J Pharmacol Exp Ther. 1984 Mar;228(3):682-90.
9
Possible role for cyclic GMP in endothelium-dependent relaxation of rabbit aorta by acetylcholine. Comparison with nitroglycerin.环磷酸鸟苷在乙酰胆碱介导的兔主动脉内皮依赖性舒张中的可能作用。与硝酸甘油的比较。
Res Commun Chem Pathol Pharmacol. 1983 Sep;41(3):369-81.
10
Role of endothelium in responses of vascular smooth muscle.内皮细胞在血管平滑肌反应中的作用。
Circ Res. 1983 Nov;53(5):557-73. doi: 10.1161/01.res.53.5.557.
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