Radomski M W, Palmer R M, Moncada S
Wellcome Research Laboratories, Langley Court, Beckenham, Kent.
Lancet. 1987 Nov 7;2(8567):1057-8. doi: 10.1016/s0140-6736(87)91481-4.
The adhesion of human platelets to monolayers of bovine endothelial cells in culture was studied to determine the role of endothelium-derived nitric oxide in the regulation of platelet adhesion. The adhesion of unstimulated and thrombin-stimulated platelets, washed and labelled with indium-111, was lower in the presence than in the absence of bradykinin or exogenous nitric oxide. The inhibitory action of both bradykinin and nitric oxide was abolished by haemoglobin, but not by aspirin, and was potentiated by superoxide dismutase to a similar degree. It is suggested that the effect of bradykinin is mediated by the release of nitric oxide from the endothelial cells, and that nitric oxide release contributes to the non-adhesive properties of vascular endothelium.
研究了人血小板与培养的牛内皮细胞单层的黏附情况，以确定内皮衍生的一氧化氮在调节血小板黏附中的作用。用铟 - 111洗涤并标记的未刺激和凝血酶刺激的血小板，在存在缓激肽或外源性一氧化氮的情况下，其黏附率低于不存在时。血红蛋白可消除缓激肽和一氧化氮的抑制作用，但阿司匹林不能，超氧化物歧化酶可将二者的抑制作用增强到相似程度。提示缓激肽的作用是通过内皮细胞释放一氧化氮介导的，且一氧化氮的释放有助于血管内皮的非黏附特性。
