Matsiota P, Blancher A, Doyon B, Guilbert B, Clanet M, Kouvelas E D, Avrameas S
Department of Physiology, School of Medicine, University of Patras, Greece.
Ann Inst Pasteur Immunol. 1988 Jan-Feb;139(1):99-108. doi: 10.1016/0769-2625(88)90134-1.
Using a panel of antigens (actin, myosin, tubulin, albumin, transferrin, peroxidase, thyroglobulin, DNA, prolactin, TNP and myelin basic protein (MBP], we have tested the antibody activity of serum and cerebrospinal fluid (CSF) from healthy individuals, patients with multiple sclerosis (MS) and individuals with other neurological diseases. No differences in the concentrations and specificities of the serum antibodies were observed among the 3 groups. In contrast, we found that MS patients often had elevated CSF antibody levels against many antigens of the panel. The MS patients with local immunoglobulin production in the central nervous system (CNS) had the highest antibody levels. Restricted antibody activity against a given antigen of the panel was not observed. Compared to the two other groups, the MS group had equivalent titres of anti-MBP antibodies in the CSF. These results suggest that, in MS, a general immune dysregulation exists which leads to a local expansion of B lymphocytes producing autoantibodies with reactivities similar to those of serum natural autoantibodies.
我们使用一组抗原（肌动蛋白、肌球蛋白、微管蛋白、白蛋白、转铁蛋白、过氧化物酶、甲状腺球蛋白、DNA、催乳素、三硝基苯和髓鞘碱性蛋白[MBP]），检测了健康个体、多发性硬化症（MS）患者以及患有其他神经系统疾病个体的血清和脑脊液（CSF）的抗体活性。在这三组中，未观察到血清抗体浓度和特异性的差异。相比之下，我们发现MS患者的脑脊液针对该组许多抗原的抗体水平常常升高。在中枢神经系统（CNS）中有局部免疫球蛋白产生的MS患者抗体水平最高。未观察到针对该组给定抗原的受限抗体活性。与其他两组相比，MS组脑脊液中的抗MBP抗体滴度相当。这些结果表明，在MS中存在普遍的免疫失调，导致产生自身抗体的B淋巴细胞在局部扩增，这些自身抗体的反应性与血清天然自身抗体相似。
