Deshmukh Dnyaneshwar B, Marathi Balram, Sudini Hari Kishan, Variath Murali T, Chaudhari Sunil, Manohar Surendra S, Rani Ch V Durga, Pandey Manish K, Pasupuleti Janila
Professor Jayashankar Telangana State Agricultural University (PJTSAU), Hyderabad, India.
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
Front Genet. 2020 Jun 10;11:514. doi: 10.3389/fgene.2020.00514. eCollection 2020.
High oleic trait, resistance to rust and late leaf spot (LLS) are important breeding objectives in groundnut. Rust and LLS cause significant economic loss, and high oleic trait is an industry preferred trait that enhances economic returns. This study reports marker-assisted selection to introgress high oleic content, resistance to LLS and rust into Kadiri 6 (K 6), a popular cultivar. The alleles for target traits were selected using linked allele-specific, simple sequence repeats and single nucleotide polymorphic markers. The Fs (384), intercrossed Fs (441), BCFs (380), BCFs (195), and BCFs (343) were genotyped to obtain desired allelic combination. Sixteen plants were identified with homozygous high oleic, LLS and rust resistance alleles in BCF, which were advanced to BCF and evaluated for disease resistance, yield governing and nutritional quality traits. Phenotyping with Near-Infrared Reflectance Spectroscopy identified three lines (BCF-76, BCF-278, and BCF-296) with >80% oleic acid. The identified lines exhibit high levels of resistance to LLS and rust diseases (score of 3.0-4.0) with preferred pod and kernel features. The selected lines are under yield testing trials in multi-locations for release and commercialization. The lines reported here demonstrated combining high oleic trait with resistance to LLS and rust diseases.
高油酸特性、抗锈病和抗晚叶斑病是花生重要的育种目标。锈病和晚叶斑病会造成重大经济损失，而高油酸特性是行业青睐的特性，可提高经济回报。本研究报道了利用标记辅助选择将高油酸含量、抗晚叶斑病和抗锈病基因导入受欢迎的品种卡迪里6号（K 6）。使用连锁等位基因特异性、简单序列重复和单核苷酸多态性标记选择目标性状的等位基因。对F2（384株）、杂交F2（441株）、回交一代（BCF1，380株）、回交一代（BCF1，195株）和回交一代（BCF1，343株）进行基因分型，以获得所需的等位基因组合。在回交一代中鉴定出16株具有纯合高油酸、抗晚叶斑病和抗锈病等位基因的植株，将其推进到回交二代并对其抗病性、产量决定因素和营养品质性状进行评估。利用近红外反射光谱进行表型分析，鉴定出三个油酸含量>80%的株系（BCF-76、BCF-278和BCF-296）。所鉴定的株系对晚叶斑病和锈病表现出高抗性（病情评分为3.0 - 4.0），具有理想的荚果和籽粒特征。所选株系正在多个地点进行产量测试试验，以待发布和商业化。本文报道的株系展示了高油酸特性与抗晚叶斑病和锈病的结合。
