Vargheese Rachel Lissy, Saravanan S, Hepziba S Juliet, Kumari S Merina Prem, Pushpam A Kavitha, Kanagarajan Selvaraju, Pillai M Arumugam
Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, India.
Department of Plant Breeding and Genetics, Rice Research Station, Tamil Nadu Agricultural University, Ambasamudram, Tamil Nadu, India.
BMC Plant Biol. 2024 Jul 31;24(1):733. doi: 10.1186/s12870-024-05387-9.
Improving the quality and shelf life of groundnut oil is one of the foremost objectives of groundnut breeding programmes. This can be achieved by marker-assisted introgression, a technique that efficiently and precisely enables breeders to develop plants with enhanced qualities. This study focused on improving the oleic acid content of an elite groundnut variety, TMV 7, by introgressing a recessive mutation responsible for the increase in oleic acid from ICG 15419. Hybridization was performed between the donor and recurrent parents to develop the F, BCF, BCF and BCF populations. Introgressed lines with increased oleic acid in the genetic background of TMV 7 were identified using allele-specific marker, F435-F, F435SUB-R and a set of SSR markers were employed to recover the genome of the recurrent parent.
With two backcrosses, a total of ten homozygous plants in the BCF population were identified with oleic acid content ranging from 54.23 to 57.72% causing an increase of 36% over the recurrent parent. Among the ten lines, the line IL-23 exhibited the highest level of recurrent parent genome recovery of 91.12%.
The phenotypic evaluation of 10 homozygous introgressed lines indicated fewer differences for all other traits under study compared to the recurrent parent, except for oleic acid and linoleic acid content confirming the genetic background of the recurrent parent. The identified lines will be subjected to multilocation trials before their commercial release.
提高花生油的品质和保质期是花生育种计划的首要目标之一。这可以通过标记辅助导入来实现，该技术能有效且精确地使育种者培育出具有优良品质的植株。本研究聚焦于通过导入来自ICG 15419的一个导致油酸增加的隐性突变，来提高优良花生品种TMV 7的油酸含量。在供体亲本和轮回亲本之间进行杂交，以培育F1、BCF1、BCF2和BCF3群体。利用等位基因特异性标记F435 - F、F435SUB - R鉴定了TMV 7遗传背景下油酸含量增加的导入系，并使用一组SSR标记来恢复轮回亲本的基因组。
经过两次回交，在BCF2群体中总共鉴定出10株纯合植株，其油酸含量在54.23%至57.72%之间，比轮回亲本增加了36%。在这10个株系中，株系IL - 23表现出最高的轮回亲本基因组恢复水平，为91.12%。
对10个纯合导入系的表型评价表明，与轮回亲本相比，除油酸和亚油酸含量外，所研究的所有其他性状差异较小，这证实了轮回亲本的遗传背景。鉴定出的株系在商业发布之前将进行多点试验。
