Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125 Hunan, China.
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Oxid Med Cell Longev. 2020 Jun 9;2020:9834813. doi: 10.1155/2020/9834813. eCollection 2020.
We investigated the effects of rapamycin (RAPA) and chloroquine (CQ) in supporting growth performance and the intestinal mucosal barrier in response to deoxynivalenol (DON) in piglets. A total of 32 healthy weaned piglets (bodyweight 7.10 ± 0.58 kg) were divided into four groups and treated daily with RAPA (1 mg/kg BW), CQ (10 mg/kg BW), or a control volume of normal saline (two groups) until the end of the experiment. After feeding a basal diet for seven days, three groups were then switched to mildewed feed containing 1 mg kg/DON for a further seven days. In contrast to the control group, DON-treated piglets showed decreased average daily gain (ADG) and daily feed intake (ADFI), as well as negatively affected intestinal morphology as indicated by villus height, crypt depth, and tight junction protein expression. A group treated with RAPA and DON showed increased intestinal autophagy, aggravated inflammatory responses, and damage to the intestinal mucosa and permeability, leading to reduced growth performance. Meanwhile, a group treated with CQ and DON showed indices comparable to the non-DON control group, with alleviated inflammatory cytokines and healthy intestinal morphology and structure. They also showed better growth performance compared to DON treatment alone. These findings have important implications for mediating autophagy against DON , as well as the potential for CQ in improving growth performance and maintaining intestinal barrier integrity in weanling piglets.
我们研究了雷帕霉素（RAPA）和氯喹（CQ）在支持生长性能和肠道黏膜屏障方面对仔猪应对脱氧雪腐镰刀菌烯醇（DON）的作用。将 32 头健康断奶仔猪（体重 7.10 ± 0.58kg）分为四组，每天分别用 RAPA（1mg/kgBW）、CQ（10mg/kgBW）或等量生理盐水（两组）处理，直到实验结束。在饲喂基础日粮 7 天后，三组仔猪切换到含有 1mgkg/DON 的霉变饲料，再继续饲喂 7 天。与对照组相比，DON 处理组仔猪的平均日增重（ADG）和日采食量（ADFI）下降，肠道形态受到负面影响，如绒毛高度、隐窝深度和紧密连接蛋白表达降低。RAPA 和 DON 处理组仔猪的肠道自噬增加，炎症反应加重，肠黏膜损伤和通透性降低，导致生长性能下降。与此同时，CQ 和 DON 处理组仔猪的各项指标与非 DON 对照组相当，炎症细胞因子减轻，肠道形态和结构健康，与 DON 单独处理组相比生长性能更好。这些发现对于介导自噬对抗 DON 以及 CQ 改善断奶仔猪生长性能和维持肠道屏障完整性具有重要意义。
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