Hong Qihua, Li Xin, Lin Qian, Shen Zhuojun, Feng Jie, Hu Caihong
College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Key Laboratory of Animal Nutrition and Feed Science (Eastern of China), Ministry of Agriculture and Rural Affairs, Hangzhou 310058, China.
Animals (Basel). 2022 Jan 27;12(3):311. doi: 10.3390/ani12030311.
This study aimed to investigate the potential effects of resveratrol (RES) on intestinal function and oxidative stress in deoxynivalenol (DON)-challenged piglets. Twenty-four healthy Duroc × Yorkshire × Landrace weaned piglets at the age of 28 ± 1 days were randomly divided into four groups with six repetitions per group. The four groups were as follows: the control group (CON), fed with a basic diet; the RES group, fed with a basal diet + 300 mg/kg RES; the DON group, fed with a basal diet containing 2.65 mg/kg DON; and the DON + RES group, fed with a basal diet containing 2.65 mg/kg DON + 300 mg/kg RES. The results showed that the growth performance and intestinal function of DON-challenged piglets were significantly decreased ( < 0.05). Compared with the DON group, the average daily feed intake of piglets in the DON + RES group was significantly increased ( < 0.05). Additionally, dietary RES ameliorated DON-induced intestinal morphology impairment, as indicated by the increased ( < 0.05) jejunal villi height and the ratio of the jejunal villi height/crypt depth. Furthermore, after the addition of RES, the activities of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in the jejunum mucosa were significantly increased, and the content of malondialdehyde (MDA) was significantly declined ( < 0.05). In addition, the level of reactive oxygen species (ROS) in the mitochondria was significantly reduced by RES, while the mitochondrial membrane potential in jejunum was significantly increased by RES ( < 0.05). However, there was no obvious difference between DON + RES and DON groups on average daily gain and the ratio of feed togain, except for the significant inhibition of average daily feed intake ( < 0.05). In conclusion, RES could effectively alleviate the DON-induced oxidative stress on weaned piglets, and reduce the damage to mitochondria and intestinal morphology, so as to improve the growth performance of piglets.
本研究旨在探讨白藜芦醇（RES）对脱氧雪腐镰刀菌烯醇（DON）攻毒仔猪肠道功能和氧化应激的潜在影响。选取24头28±1日龄健康的杜洛克×约克夏×长白断奶仔猪，随机分为四组，每组6个重复。四组分别为：对照组（CON），饲喂基础日粮；RES组，饲喂基础日粮+300 mg/kg RES；DON组，饲喂含2.65 mg/kg DON的基础日粮；DON+RES组，饲喂含2.65 mg/kg DON+300 mg/kg RES的基础日粮。结果表明，DON攻毒仔猪的生长性能和肠道功能显著降低（P<0.05）。与DON组相比，DON+RES组仔猪的平均日采食量显著增加（P<0.05）。此外，日粮RES改善了DON诱导的肠道形态损伤，表现为空肠绒毛高度增加（P<0.05）以及空肠绒毛高度与隐窝深度比值增加。此外，添加RES后，空肠黏膜中超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）显著增加，丙二醛（MDA）含量显著下降（P<[0.05]）。另外，RES显著降低了线粒体中活性氧（ROS）水平，同时显著增加了空肠线粒体膜电位（P<0.05）。然而，除了平均日采食量受到显著抑制（P<0.05）外，DON+RES组与DON组在平均日增重和料重比方面没有明显差异。综上所述，RES可有效减轻DON对断奶仔猪的氧化应激，减少对线粒体和肠道形态的损伤，从而提高仔猪的生长性能。
