Koohpeima Fatemeh, Derakhshan Maryam, Mokhtari Mohammad Javad
Department of Operative Dentistry, Biomaterial Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Young Researchers and Elite Club, Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
Int J Mol Cell Med. 2019 Fall;8(4):294-300. doi: 10.22088/IJMCM.BUMS.8.4.294.
Dental decay is a disease that is greatly affected by environmental components, but recently there have been an increasing number of documents supporting a genetic factor in the development of caries. The purpose of this study was to examine the association between dental caries and single-nucleotide polymorphisms in the gene. This research was carried out on 360 individuals of both sexes, who were referred to the dental school at the Shiraz University of Medical Sciences. In this research, individuals aged 20-65 years were divided into two groups: controls (decayed, missed, or filled teeth (DMFT) ≤ 5; n = 180) and cases (DMFT ≥ 14; n = 180). The tetra-primer ARMS-PCR technique was performed for genotyping the DNA extracted from blood cells. Analysis of the rs946252 polymorphism showed that the T allele of rs946252 was a significant protective factor against dental caries in Iranian adults (T vs. C: OR = 0.70, 95% CI: 0.49-0.98, P = 0.04). We demonstrated the significant differences in the genotype frequencies under two genetic models: overdominant (TC vs. TT + CC: OR 0.35, 95% CI 0.19-0.64, P = 0.0006) and recessive (CC vs. TC + TT: OR 2.57, 95% CI 1.39-4.76, P = 0.002). Our results show that the SNPs of the gene may be related with susceptibility to dental caries in Iranian adults.
龋齿是一种受环境因素影响很大的疾病，但最近有越来越多的文献支持龋齿发生发展中的遗传因素。本研究的目的是探讨龋齿与该基因单核苷酸多态性之间的关联。本研究对360名男女个体进行，这些个体均来自设拉子医科大学牙科学院。在本研究中，年龄在20 - 65岁的个体被分为两组：对照组（龋失补牙数（DMFT）≤5；n = 180）和病例组（DMFT≥14；n = 180）。采用四引物ARMS - PCR技术对从血细胞中提取的DNA进行基因分型。对rs946252多态性的分析表明，rs946252的T等位基因是伊朗成年人患龋齿的显著保护因素（T vs. C：比值比 = 0.70，95%可信区间：0.49 - 0.98，P = 0.04）。我们在两种遗传模型下证明了基因型频率存在显著差异：超显性模型（TC vs. TT + CC：比值比0.35，95%可信区间0.19 - 0.64，P = 0.0006）和隐性模型（CC vs. TC + TT：比值比2.57，95%可信区间1.39 - 4.76，P = 0.002）。我们的结果表明，该基因的单核苷酸多态性可能与伊朗成年人患龋齿的易感性有关。
