Department of Oral Pathology and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
Oral Dis. 2011 May;17(4):399-406. doi: 10.1111/j.1601-0825.2010.01766.x. Epub 2010 Nov 29.
Dental caries is greatly influenced disease by environmental factors, but recently there are increasing evidences for a genetic component in caries susceptibility. AMELX is the gene coding amelogenin, which is the most important factor for normal enamel development. The aim of this study was to examine the relationship between dental caries and single nucleotide polymorphisms (SNPs) in AMELX.
For this study, we used DNA samples collected from 120 unrelated individuals older than 12 years of age. All of them were examined for their oral and dental status under the WHO recommended criteria, and clinical information such as DMFT and DMFS were evaluated. Individuals whose DMFT and DMFS index lower than 2 were designated 'very low caries experience' and higher than 3 were designated 'higher caries experience'. Genomic DNA was extracted from hair samples, and single nucleotide polymorphisms of AMELX were genotyped. Genotyping of three SNPs (rs17878486, rs5933871, rs5934997, intron) in AMELX gene was determined by direct sequencing and analyzed with SNPStats.
There were significant associations between rs5933871 and rs5934997 SNP and caries susceptibility in the water fluoridation group.
These results suggest that SNPs of AMELX might be associated with dental caries susceptibility in Korean population.
龋齿受环境因素影响较大，但最近有越来越多的证据表明龋齿易感性存在遗传成分。AMELX 是编码釉原蛋白的基因，釉原蛋白是正常釉质发育的最重要因素。本研究旨在探讨 AMELX 中单核苷酸多态性 (SNP) 与龋齿之间的关系。
本研究使用了 120 名年龄在 12 岁以上的无血缘关系个体的 DNA 样本。根据世界卫生组织推荐的标准对所有个体进行口腔和牙齿状况检查，并评估 DMFT 和 DMFS 等临床信息。DMFT 和 DMFS 指数低于 2 的个体被指定为“极低龋齿经历”，高于 3 的个体被指定为“高龋齿经历”。从毛发样本中提取基因组 DNA，并对 AMELX 的单核苷酸多态性进行基因分型。通过直接测序确定 AMELX 基因中三个 SNP(rs17878486、rs5933871、rs5934997、内含子)的基因分型，并使用 SNPStats 进行分析。
在水氟化物组中，rs5933871 和 rs5934997 SNP 与龋齿易感性之间存在显著关联。
这些结果表明，AMELX 的 SNP 可能与韩国人群的龋齿易感性有关。
