慢性中耳炎培养阳性患者自噬标志物表达降低。
Decreased expression of autophagy markers in culture-positive patients with chronic otitis media.
作者信息
Jung Junyang, Park Dong Choon, Kim Young Il, Lee Eun Hye, Park Myung Jin, Kim Sang Hoon, Yeo Seung Geun
机构信息
Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul, Korea.
St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.
出版信息
J Int Med Res. 2020 Jun;48(6):300060520936174. doi: 10.1177/0300060520936174.
OBJECTIVE
Abnormal autophagy plays a role in the pathogenesis of various diseases. This study aimed to evaluate associations between the clinical manifestations of chronic otitis media (COM) and expression of autophagy markers.
METHODS
Associations between presence of bacteria, otorrhea, and conductive and sensorineural hearing loss and levels of autophagy-related mRNAs were investigated in 47 patients with COM.
RESULTS
Autophagy-related mRNAs were detected in all inflammatory tissues of COM patients. LC3-II showed the highest level of expression, followed by Beclin-1, P13KC3, Rubicon, and mTOR. Beclin-1 mRNA levels were significantly lower in culture-positive than in culture-negative patients.
CONCLUSION
Autophagy is involved in the pathogenesis of COM. The finding that expression of autophagy markers, especially Beclin-1, was lower in culture-positive than in culture-negative patients suggested that these markers are closely associated with the clinical features of COM.
目的
异常自噬在多种疾病的发病机制中起作用。本研究旨在评估慢性中耳炎（COM）的临床表现与自噬标志物表达之间的关联。
方法
在47例COM患者中，研究细菌的存在、耳漏以及传导性和感音神经性听力损失与自噬相关mRNA水平之间的关联。
结果
在COM患者的所有炎症组织中均检测到自噬相关mRNA。LC3-II表达水平最高，其次是Beclin-1、P13KC3、Rubicon和mTOR。培养阳性患者的Beclin-1 mRNA水平显著低于培养阴性患者。
结论
自噬参与COM的发病机制。培养阳性患者的自噬标志物，尤其是Beclin-1的表达低于培养阴性患者，这一发现表明这些标志物与COM的临床特征密切相关。
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