Deng Ju, Huang Yan, Liang Jingjie, Jiang Yuewen, Chen Tiansheng
State Key Laboratory of Mariculture Breeding, Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Jimei University, Xiamen 361021, China.
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, China.
Animals (Basel). 2024 Aug 19;14(16):2406. doi: 10.3390/ani14162406.
Research across various species has demonstrated that the doublesex and mab-3-related transcription factor 3 () plays pivotal roles in testis development. However, the precise molecular mechanisms of remain unclear. In this study, we investigated the role of () in testis development using the model organism medaka (). SqRT-PCR and ISH analyses revealed that is predominantly expressed in the testis, especially in the spermatid and spermatozoon. Using CRISPR/Cas9, we generated two homozygous mutants (-8 bp and -11 bp), which exhibited significantly reduced fertilization rates and embryo production. Additionally, the number of germ cells and sperm motility were markedly decreased in the mutants, manifesting as the symptoms of asthenozoospermia and oligozoospermia. Interestingly, RNA-Seq analysis showed that the deficiency of could lead to a significant downregulation of numerous genes related to gonadal development and severe disruptions in mitochondrial function. These results suggested that is essential for spermatogenesis and spermatozoa energy production. This paper provides new insights and perspectives for further exploring the molecular mechanisms underlying spermatogenesis and addressing male reproductive issues.
对多种物种的研究表明，双性和mab - 3相关转录因子3（）在睾丸发育中起关键作用。然而，其确切的分子机制仍不清楚。在本研究中，我们利用模式生物青鳉（）研究了（）在睾丸发育中的作用。SqRT - PCR和ISH分析表明，主要在睾丸中表达，尤其是在精子细胞和精子中。利用CRISPR/Cas9，我们产生了两个纯合突变体（- 8bp和- 11bp），它们的受精率和胚胎产量显著降低。此外，突变体中的生殖细胞数量和精子活力明显下降，表现为弱精子症和少精子症的症状。有趣的是，RNA - Seq分析表明，的缺失会导致许多与性腺发育相关的基因显著下调，并严重破坏线粒体功能。这些结果表明，对精子发生和精子能量产生至关重要。本文为进一步探索精子发生的分子机制和解决男性生殖问题提供了新的见解和观点。
